FlyBase Gene Report: Dmel\qua

General Information

Symbol

Dmel\qua

Species

D. melanogaster

Name

quail

Annotation Symbol

CG6433

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003187

Gene Model Status

Current

Stock Availability

19 publicly available

Gene Summary

quail (qua) encodes a member of the Villin family of F-actin crosslinking proteins. It is involved in wing hair growth and oogenesis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Also Known As

villin

Key Links

Genomic Location

Cytogenetic map

36C10-36C10

Sequence location

2L:17,486,806..17,497,208 [-]

Recombination map

2-53

RefSeq locus

NT_033779 REGION:17486806..17497208

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables actin binding

inferred from direct assay

(Mahajan-Miklos and Cooley, 1994)

inferred from mutant phenotype

(Mahajan-Miklos and Cooley, 1994)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables actin binding

inferred from sequence or structural similarity with FLYBASE:Gel; FB:FBgn0010225

(Mahajan-Miklos and Cooley, 1994)

inferred from sequence or structural similarity with UniProtKB:P09327

(Mahajan-Miklos and Cooley, 1994)

inferred from electronic annotation with InterPro:IPR003128, InterPro:IPR036886

(InterPro Project Members, 2004-)

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN002633351

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR007122

(InterPro Project Members, 2004-)

enables calcium ion binding

inferred from sequence or structural similarity with UniProtKB:P09327

(Mahajan-Miklos and Cooley, 1994)

enables phosphatidylinositol-4,5-bisphosphate binding

inferred from biological aspect of ancestor with PANTHER:PTN000240164

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in imaginal disc-derived wing hair organization

inferred from mutant phenotype

(Guild et al., 2005)

involved_in ovarian nurse cell to oocyte transport

inferred from high throughput mutant phenotype

(Schupbach and Wieschaus, 1991)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in actin filament severing

inferred from biological aspect of ancestor with PANTHER:PTN000240164

(GO Reference Genome Project, 2011-)

involved_in actin polymerization or depolymerization

inferred from biological aspect of ancestor with PANTHER:PTN008531885

(GO Reference Genome Project, 2011-)

involved_in barbed-end actin filament capping

inferred from biological aspect of ancestor with PANTHER:PTN000240164

(GO Reference Genome Project, 2011-)

involved_in cytoskeleton organization

inferred from electronic annotation with InterPro:IPR003128, InterPro:IPR036886

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

colocalizes_with actin filament

inferred from direct assay

(Mahajan-Miklos and Cooley, 1994)

located_in cytoplasm

inferred from direct assay

(Mahajan-Miklos and Cooley, 1994)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in actin cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN002633351

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the villin/gelsolin family. (Q23989)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

quail (qua) encodes a member of the Villin family of F-actin crosslinking proteins. It is involved in wing hair growth and oogenesis. [Date last reviewed: 2019-03-14]

Protein Function (UniProtKB)

Required for the formation of cytoplasmic actin filament bundles in nurse cells, possibly by regulating both the polymerization and organization of actin filaments. Mutations in quail result in female sterility due to the disruption of cytoplasmic transport from the nurse cells into the oocyte late in oogenesis.

(UniProt, Q23989)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

fs(2)A12

Homozygous females anatomically normal; lay foreshortened eggs with truncated anterior tip and short fat chorionic filaments. Eggs similar to stage-11 oocytes, some with adhering follicle and nurse cells. A few eggs lack stainable chromatin; most have one to three large spherical masses of fragmented chromosomes or tripolar spindles and abnormally elongated mitotic figures.

qua: quail (T. Schupbach)

Female sterile; nurse-cell contents not transported into the egg; follicle cells produce normal chorion around tiny eggs, which remain unfertilized.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\qua for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q23989)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

qua-RA

FBtr0080996

NM_165222

3266

887

qua-RB

FBtr0080997

NM_165223

3306

888

qua-RC

FBtr0306165

NM_001259137

3318

892

Additional Transcript Data and Comments

Reported size (kB)

3.3 (northern blot)

(Mahajan-Miklos and Cooley, 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

qua-PA

FBpp0080549

100.9

887

7.96

NP_724060

AAF53623

qua-PB

FBpp0080550

101.0

888

7.70

FBpp0297295

101.5

892

7.84

M9NF69

NP_001246066

AFH03740

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

887 (aa); 97 (kD observed); 97 (kD predicted)

(Mahajan-Miklos and Cooley, 1994)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\qua using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

qua_1
qua_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.58

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic dorsal epidermis

(Zhai et al., 2010)

germline cell

(Zhai et al., 2010)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

germline cell

(Fisher et al., 2012)

embryonic stage 10 -- 11

posterior spiracle primordium | anterior

(Zhai et al., 2010)

embryonic stage 11 -- 12

dorsal epidermis primordium

(Fisher et al., 2012)

gnathal primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 9

Comment: reference states 0-4 hr AEL

(Mahajan-Miklos and Cooley, 1994)

pupal stage

(Mahajan-Miklos and Cooley, 1994)

adult stage | female

ovary

(Mahajan-Miklos and Cooley, 1994)

adult stage & oogenesis

oocyte

(Mahajan-Miklos and Cooley, 1994)

nurse cell

(Mahajan-Miklos and Cooley, 1994)

spermatogenesis & adult stage

testis

(Mahajan-Miklos and Cooley, 1994)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

border follicle cell

(Borghese et al., 2006)

Additional Descriptive Data

qua is enriched in border follicle cells relative to follicle cells.

(Borghese et al., 2006)

qua transcripts are abundant in male testes and female ovaries and in early embyros and are detected at very low levels at other times of development by northern blots. qua transcripts are detected in nurse cells and in the oocyte by in situ hybridization.

(Mahajan-Miklos and Cooley, 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

nurse cell

(Mahajan-Miklos and Cooley, 1994)

ring canal

(Mahajan-Miklos and Cooley, 1994)

oocyte

(Mahajan-Miklos and Cooley, 1994)

oogenesis

border follicle cell

(Borghese et al., 2006)

Additional Descriptive Data

In oocyte stages S8-S10A, qua protein colocalizes with the subcortical actin filament network in the nurse cells and the oocyte. It is also present in the cytoplasm of nurse cells. Occasionally, it localizes to ring canals. In stage S10B, the levels of qua protein rise significantly and it is found along the length of the newly assembled actin filament bundles in the nurse cell cytoplasm.

(Borghese et al., 2006, Mahajan-Miklos and Cooley, 1994)

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with actin filament

inferred from direct assay

(Mahajan-Miklos and Cooley, 1994)

located_in cytoplasm

inferred from direct assay

(Mahajan-Miklos and Cooley, 1994)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\qua in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\qua

View exonic expression by tissue for Dmel\qua

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003187

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35058/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003187

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003187

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003187

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6433

Flygut - An atlas of the Drosophila adult midgut

FBgn0003187

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\qua

Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 37 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of qua

Transgenic constructs containing regulatory region of qua

Aberrations (Deficiencies and Duplications) ( 17 )

Inferred from experimentation ( 17 )

Gene not disrupted in

Df(2L)Gpdh1-A

(Bloomington Drosophila Stock Center, 1998.9.22)

Df(2L)M36F-S5

(Bloomington Drosophila Stock Center, 1998.9.22, Tearle and Nusslein-Volhard, 1987, Steward and Nusslein-Volhard, 1986)

Df(2L)TW201

(Steward and Nusslein-Volhard, 1986)

Df(2L)TW202

(Steward and Nusslein-Volhard, 1986)

Df(2L)TW203

(Steward and Nusslein-Volhard, 1986)

Df(2L)TW330

(Steward and Nusslein-Volhard, 1986)

Df(2L)VA18

(Steward and Nusslein-Volhard, 1986)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

qua^EY03072

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

qua^GD11926

viable, with Scer\GAL4^Mef2.PR

qua^GD11926

viable, with Scer\GAL4^pnr-MD237

qua^GD11926

Sterility

Allele

female sterile

female sterile | recessive

female sterile | recessive | heat sensitive

qua^WP614

fertile

qua^EY03072

Other Phenotypes

Allele

abnormal mitotic cell cycle | recessive

decreased size | adult stage, with Scer\GAL4^hh-Gal4

qua^KK103765

decreased size | larval stage, with Scer\GAL4^ap-md544

qua^KK103765

decreased size | larval stage, with Scer\GAL4^hh-Gal4

qua^KK103765

wild-type

Phenotype manifest in

Allele

actin filament & egg chamber

qua¹

actin filament bundle | oogenesis (with qua^6-396)

qua^8-1062

actin filament bundle | oogenesis (with qua^8-1062)

qua^6-396

dorsal appendage

egg

egg chamber

epithelial cell of wing disc, with Scer\GAL4^ap-md544

qua^KK103765

epithelial cell of wing disc, with Scer\GAL4^hh-Gal4

qua^KK103765

extracellular vesicle | decreased number | larval stage, with Scer\GAL4^hh-Gal4

qua^KK103765

female reproductive system

microvillus | decreased number | larval stage, with Scer\GAL4^ap-md544

qua^KK103765

microvillus | decreased number | larval stage, with Scer\GAL4^hh-Gal4

qua^KK103765

nucleolus | oogenesis (with qua^6-396)

qua^8-1062

nucleolus | oogenesis (with qua^8-1062)

qua^6-396

nurse cell

qua¹

nurse cell & actin filament

nurse cell | oogenesis (with qua^6-396)

qua^8-1062

nurse cell | oogenesis (with qua^8-1062)

qua^6-396

nurse cell ring canal

qua¹

oocyte

ovary

spindle

stretch follicle cell

wing, with Scer\GAL4^hh-Gal4

qua^KK103765

wing disc, with Scer\GAL4^ap-md544

qua^KK103765

wing disc, with Scer\GAL4^hh-Gal4

qua^KK103765

wing hair (with qua¹)

qua^6-396

wing hair (with qua^6-396)

qua¹

wing pouch, with Scer\GAL4^ap-md544

qua^KK103765

wing pouch, with Scer\GAL4^hh-Gal4

qua^KK103765

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (13)

Hsap\AVIL

5 of 14

Yes

Hsap\GSN

5 of 14

Yes

Hsap\SCIN

5 of 14

Yes

Hsap\VIL1

5 of 14

Yes

Hsap\VILL

5 of 14

Yes

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

Rnor\Avil

5 of 14

Yes

Rnor\Gsn

5 of 14

Yes

Rnor\Scin

5 of 14

Yes

Rnor\Vil1

5 of 14

Yes

Rnor\Vill

5 of 14

Yes

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Mus musculus (laboratory mouse) (14)

Mmus\Avil

5 of 14

Yes

Mmus\Gsn

5 of 14

Yes

Mmus\Scin

5 of 14

Yes

Mmus\Vill

5 of 14

Yes

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (17)

Xtro\capg

3 of 13

Yes

Xtro\gsn

3 of 13

Yes

Xtro\vil1

3 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\nek6

1 of 13

Xtro\phlpp1

1 of 13

Xtro\podn

1 of 13

Yes

Xtro\rundc3a

1 of 13

Yes

Xtro\scin

1 of 13

Xtro\svil

1 of 13

Xtro\vill

1 of 13

Danio rerio (Zebrafish) (20)

Drer\gsna

5 of 14

Yes

Drer\gsnb

5 of 14

Yes

Drer\scin

5 of 14

Yes

Drer\scinlb

5 of 14

Yes

Drer\vil1

5 of 14

Yes

Drer\vill

5 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\gsnl-1

6 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP009232

12 of 12

Yes

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (30)

Atha\VLN1

6 of 13

Yes

Atha\VLN2

6 of 13

Yes

Atha\VLN3

6 of 13

Yes

Atha\VLN4

6 of 13

Yes

Atha\VLN5

6 of 13

Yes

Atha\AT1G72180

1 of 13

Atha\AT3G47090

1 of 13

Yes

Atha\AT3G47110

1 of 13

Yes

Atha\AT3G47570

1 of 13

Yes

Atha\AT3G47580

1 of 13

Yes

Atha\AT3G51560

1 of 13

Yes

Atha\AT4G26540

1 of 13

Yes

Atha\AT5G18350

1 of 13

Yes

Atha\BRL1

1 of 13

Atha\CLV1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\RLP1

1 of 13

Atha\RLP9

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:qua. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

Gel

5 of 13

fliI

4 of 13

Svil

3 of 13

chp

1 of 13

Unc-115a

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

AVIL; advillin

model of nephrotic syndrome type 21

IEA

(FlyBase, 2019-)

GSN; gelsolin

model of Finnish type amyloidosis

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

AVIL; advillin

5 of 14

613397

NEPHROTIC SYNDROME, TYPE 21; NPHS21

nephrotic syndrome type 21

GSN; gelsolin

5 of 14

137350

AMYLOIDOSIS, FINNISH TYPE

Finnish type amyloidosis

5 of 14

5 of 14

5 of 14

CAPG; capping actin protein, gelsolin like

4 of 14

153615

SVIL; supervillin

3 of 14

604126

MYOFIBRILLAR MYOPATHY 10; MFM10

myofibrillar myopathy 10

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

qua - cnn

anti bait coimmunoprecipitation, peptide massfingerprinting

(Habermann et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

qua

suppressible

spoon

(Neuman-Silberberg, 2007)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

CycB

suppressible

qua

(Ji et al., 2002)

suppressible

qua

(Cant et al., 1998)

enhanceable

qua

(Cant et al., 1998)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61054

DroID - A comprehensive database of gene and protein interactions.

FBgn0003187

MIST (genetic) - An integrated Molecular Interaction Database

35058

MIST (protein-protein) - An integrated Molecular Interaction Database

35058

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-53

Cytogenetic map

36C10-36C10

Sequence location

2L:17,486,806..17,497,208 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36C10-36C10

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-53

(FlyBase, 2016, Tearle and Nusslein-Volhard, 1987)

2-52.7

(Comeron et al., 2012)

(Bakken, 1973)

Left of (cM)

Right of (cM)

(Tearle and Nusslein-Volhard, 1987)

Notes

Stocks and Reagents

Stocks (19)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4571

qua^6-396/SM1

4572

qua^8-1062/SM1

41856

y¹ v¹; P{TRiP.GL01284}attP2

43817

y¹ w^*; Mi{MIC}qua^MI07365

61046

y¹ w^*; Mi{MIC}qua^MI15417

Kyoto

107801

qua^6-396/SM1

107802

qua^8-1062/CyO

125527

w¹¹¹⁸; P{RS5}qua^5-HA-2017

NIG-Fly

6433R-3

P{NIG.6433R}3

HMJ23789

y¹ v¹; P{TRiP.HMJ23789}attP40/CyO

VDRC

v100856

P{KK103765}VIE-260B

v27623

w¹¹¹⁸; P{GD11926}v27623

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (12)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE36860

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003187

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003187

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Mahajan-Miklos and Cooley, 1994)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

qua protein can bind and bundle filamentous actin in vitro, regardless of the concentration of calcium ions.

(Matova et al., 1999)

Like vertebrate villin, qua product can bundle actin filaments in vitro.

(Knowles et al., 1995)

The villin-like product of the qua locus is required for the formation of cytoplasmic actin filament bundles in nurse cells, possibly by regulating both the polymerization and organization of actin filaments as has been demonstrated for vertebrate villin in vitro.

(Mahajan-Miklos and Cooley, 1994)

Mutations at the qua locus cause defects in midoogenesis.

(Schupbach and Wieschaus, 1991)

Relationship to Other Genes

Source for database merge of

Source for merge of: qua fs(2)A12

(Bloomington Drosophila Stock Center, 1998.9.22)

Source for merge of: qua anon-WO0140519.71

(FlyBase, 1996-)

Additional comments

Source for merge of qua anon-WO0140519.71 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: qua CG6433

(Zhong, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Name Synonyms

Quail

(Rodal et al., 2015, Huelsmann et al., 2013, Bosveld et al., 2008, Lerit and Gavis, 2007, Rogers et al., 2003)

Villin

(Huelsmann et al., 2013)

female sterile(2)A12

villin

(Fabian and Brill, 2012, Noguchi et al., 2008, Guild et al., 2005, Tilney et al., 2004, Starr and Han, 2003)

Secondary FlyBase IDs

FBgn0000942
FBgn0044556

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 57 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35058

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q23989

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q23989

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9NF69

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q23989

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003187

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35058/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003187

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003187

FlyMine - An integrated database for Drosophila genomics

FBgn0003187

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6433

MARRVEL_MODEL - MARRVEL (model organism gene)

35058

Linkouts

BioGRID - A database of protein and genetic interactions.

61054

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003187

DroID - A comprehensive database of gene and protein interactions.

FBgn0003187

DRSC - Results frm RNAi screens

FBgn0003187

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

qua_1
qua_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003187

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003187

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6433

Flygut - An atlas of the Drosophila adult midgut

FBgn0003187

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003187

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013108

MIST (genetic) - An integrated Molecular Interaction Database

35058

MIST (protein-protein) - An integrated Molecular Interaction Database

35058

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (132)

The following external sites may use different assemblies or annotations than FlyBase.

2.58

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