Expression of holn1^GD11573 using Scer\GAL4^Bx-MS1096 results in a range of phenotypes, from wild-type looking to a smaller, cylindrically curved wing with an increased number of veins, or a blistered wing with much of the surface converted to vein material.

Expression of holn1^GD11573 using Scer\GAL4^Act5C.PU results in late pupal lethality. These late pupae often have extra and/or misplaced or missing macrochaetae on the scutellum.

Adults expressing holn1^GD11573 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 50-60% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.