FlyBase Allele Report: Dmel\Oga[GD10644]

General Information

Symbol

Dmel\Oga^GD10644

Species

D. melanogaster

Name

FlyBase ID

FBal0204856

Feature type

allele

Associated gene

Dmel\Oga

Associated Insertion(s)

Carried in Construct

P{GD10644}

Also Known As

UAS-OgaRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10644}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10644

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^tim.UAS

(Kaasik et al., 2013)

increased body size | adult stage, with Scer\GAL4^Ilp2.PR

(Sekine et al., 2010)

increased body size | third instar larval stage, with Scer\GAL4^Ilp2.PR

(Sekine et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Oga^GD10644 under the control of Scer\GAL4^tim.Scer\UAS results in lengthening of the period of the free-running locomotor activity rhythm in constant darkness.

(Kaasik et al., 2013)

Adults expressing Oga^GD10644 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Third instar larvae and adults expressing Oga^GD10644 under the control of Scer\GAL4^Ilp2.PR have a significantly increased body size compared to controls.

Expression of Oga^GD10644 under the control of Scer\GAL4^Ilp2.PR is not overtly toxic to insulin-producing cells (IPCs); the IPC nuclei in third larval instar brains do not show any changes that would be consistent with apoptosis or necrosis.

Hemolymph carbohydrate levels are increased in third instar larvae and adults expressing Oga^GD10644 under the control of Scer\GAL4^Ilp2.PR compared to controls.

Expression of Oga^GD10644 under the control of Scer\GAL4^Lsp2.PH reduces the accumulation of neutral lipids in the fat body compared to controls.

(Sekine et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development