FlyBase Allele Report: Dmel\Idua[GD5261]

General Information

Symbol

Dmel\Idua^GD5261

Species

D. melanogaster

Name

FlyBase ID

FBal0204990

Feature type

allele

Associated gene

Dmel\Idua

Associated Insertion(s)

Carried in Construct

P{GD5261}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5261}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5261

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of mucopolysaccharidosis I

CEA

(De Filippis et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(De Filippis et al., 2021)

abnormal locomotor behavior | adult stage, with Scer\GAL4^repo

(De Filippis et al., 2021)

lethal | male limited, with Scer\GAL4^Act5C.PI

(De Filippis et al., 2021)

lethal - all die before end of P-stage, with Scer\GAL4^Mef2.PR

(De Filippis et al., 2021)

lethal - all die before end of P-stage, with Scer\GAL4^αTub84B.PL

(De Filippis et al., 2021)

long lived, with Scer\GAL4^elav-C155

(De Filippis et al., 2021)

long lived, with Scer\GAL4^repo

(De Filippis et al., 2021)

partially lethal - majority die, with Scer\GAL4^Act5C.PI

(De Filippis et al., 2021)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal - majority live, with Scer\GAL4^repo

(De Filippis et al., 2021)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^elav-C155

(De Filippis et al., 2021)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Idua^GD5261 under the control of Scer\GAL4^elav-C155 or Scer\GAL4^repo leads to decreased climbing ability in adults, as compared to controls.

The expression of Idua^GD5261 under the control of Scer\GAL4^αTub84B.PL leads to third instar larvae show significant increases in the number and size of lysosomes in the ventral nerve cord.

The expression of Idua^GD5261 under the control of Scer\GAL4^Mef2.PR leads to third instar larval muscles with significant increases in the number and size of lysosomes, as well as in the number of autophagosomes under fed conditions, but not under starvation conditions; the proportion of autophagosomes that fuse to lysosomes is drastically diminished under fed conditions, but not under starvation conditions, as compared to controls.

(De Filippis et al., 2021)

Adults expressing CG6201^GD5261 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5261

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v13244

w¹¹¹⁸; P{GD5261}v13244

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5261

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG6201^GD5261

Idua^GD5261

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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