FlyBase Allele Report: Dmel\BHD[GD6590]

General Information

Symbol

Dmel\BHD^GD6590

Species

D. melanogaster

Name

FlyBase ID

FBal0205937

Feature type

allele

Associated gene

Dmel\BHD

Associated Insertion(s)

Carried in Construct

P{GD6590}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6590}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6590

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased body size, with Scer\GAL4^Tub.PU

(Gaur et al., 2013)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

nucleolus, with Scer\GAL4^Tub.PU

(Gaur et al., 2013)

Detailed Description

Statement

Reference

Expression of BHD^GD6590 under the control of Scer\GAL4^tub.PU results in an increase in nucleolar volume in larval salivary gland cells. In addition, the nucleoli are multi-lobed (in contrast to the round nucleolus seen in wild type).

Adults expressing BHD^GD6590 under the control of Scer\GAL4^tub.PU are larger in size and weigh more than wild-type controls.

(Gaur et al., 2013)

Adults expressing BHD^GD6590 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6590

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of BHD^GD6590 enhances the semi-lethality caused by expression of Ras85D^V12.Scer\UAS under the control of Scer\GAL4^ey.PU at 21-22[o]C, such that few adult escapers emerge. The eye defects of adult escapers are enhanced.

Co-expression of BHD^GD6590 exacerbates the increase in the number of mitotic cells per eye imaginal disc seen in larvae expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^ey.PU.

(Gaur et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

BHD^GD6590 is rescued by Scer\GAL4^Tub.PU/BHD^UAS.Tag:HA

(Gaur et al., 2013)

Comments

Co-expression of BHD^{Scer\UAS.T:Ivir\HA1} suppresses the increase in nucleolar volume seen in salivary gland cells of larvae expressing BHD^GD6590 under the control of Scer\GAL4^tub.PU.

(Gaur et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v38248

w¹¹¹⁸; P{GD6590}v38248

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts