Expressing CCT3^GD14084 under the control of Scer\GAL4^ey.PU results in complete loss of head structures in pharate adults.

Expressing CCT3^GD14084 under the control of Scer\GAL4^GMR.PU fails to induce cell death in the posterior compartment of the eye disc in third instar larvae, and results in adults with normal eyes.

The expression of CCT3^GD14084 under the control of either Scer\GAL4^tin.CΔ4 or Scer\GAL4^HCH.Hand leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to a severe decrease in lifespan and leads to near or fully penetrant cardiac defects, respectively, namely morphological defects, non-beating heart segments and non-beating hearts, as compared to controls. Expression under the control of Scer\GAL4^tin.CΔ4 also leads to progressive cardiac dysfunction, as shown by significant increases in heart period, arrhythmia, and diastolic and systolic intervals, and by significant decreases in fractional shortening, and diastolic and systolic diameter, as compared to controls.

The expression of CCT3^GD14084 under the control of Scer\GAL4^ey.PH leads to rough eye phenotype, with reduced number of ommatidia, as compared to controls; expression under the control of Scer\GAL4^elav-C155 leads to the neuromuscular junctions of third instar larval muscles 6/7 exhibiting decreases in the size and number of terminal boutons, as compared to controls.

Adults expressing Cctγ^GD14084 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late larval lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.