FlyBase Allele Report: Dmel\Fim[GD1609]

General Information

Symbol

Dmel\Fim^GD1609

Species

D. melanogaster

Name

FlyBase ID

FBal0207035

Feature type

allele

Associated gene

Dmel\Fim

Associated Insertion(s)

Carried in Construct

P{GD1609}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1609}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1609

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased size | adult stage, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

decreased size | larval stage, with Scer\GAL4^ap-md544

(Hurbain et al., 2022)

decreased size | larval stage, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

epithelial cell of wing disc, with Scer\GAL4^ap-md544

(Hurbain et al., 2022)

epithelial cell of wing disc, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

extracellular vesicle | decreased number | larval stage, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

microvillus | decreased number | larval stage, with Scer\GAL4^ap-md544

(Hurbain et al., 2022)

microvillus | decreased number | larval stage, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

wing, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

wing disc, with Scer\GAL4^ap-md544

(Hurbain et al., 2022)

wing disc, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

wing pouch, with Scer\GAL4^ap-md544

(Hurbain et al., 2022)

wing pouch, with Scer\GAL4^hh-Gal4

(Hurbain et al., 2022)

Detailed Description

Statement

Reference

The expression of Fim^GD1609 under the control of Scer\GAL4^hh-Gal4 or Scer\GAL4^ap-md544 induces a small wing disc pouch, as well as fewer and shortened/misshapen in microvilli in the wing disc posterior compartment. In the Scer\GAL4^hh-Gal4-driven expression condition, wing discs show a decrease in the number of extracellular vesicle; adults show a small wing.

(Hurbain et al., 2022)

Adults expressing Fim^GD1609 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Fim^GD1609 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Fim^GD1609 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD1609} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development