UASt regulatory sequences drive expression of an inverted repeat.
Expression of Gr59fGD628 under the control of Scer\GAL4Gr33a-GAL4 has no impact on the L-canavanine avoidance seen in wild type flies in a two-way choice behavioral assay.
Adults expressing Gr59fGD628 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.
Adults expressing Gr59fGD628 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or late larval lethality.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.