Class I neuron ddaE neurons expressing Klp61F^GD14149 under the control of Scer\GAL4²²¹ (together with Dicer-2 for a more efficient RNAi) show a higher rate of cell body-directed growing microtubules to successfully exit a distal or peripheral branch point, but not a proximal branch point; microtubule bundles form triangular arrangement of at dendrite branch points.

At 18[o]C, nearly all animals expressing Klp64D^GD7048 under the control of Scer\GAL4^nub-AC-62 show mild defects in the distal part of the wings. Most of the defects seen comprise notching in the wing margin and loss of margin bristles. Phenotypes are temperature-dependent: at 29[o]C, flies can almost develop to the adult but die in the pupal case. These flies show severe shrinkage or complete loss of wing.

Expressing Klp64D^GD7048 at and near the DV boundary region using Scer\GAL4^bbg-C96 causes notching along the wing margin. This phenotype is seen with 100% penetrance.

Expression of Klp64D^GD7048 under the control of Scer\GAL4^elav.PLu results in a failure to initiate axon regeneration after injury.

Adults expressing Klp64D^GD7048 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Klp64D^GD7048 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD7048} insertion line used.