FlyBase Allele Report: Dmel\Msp300[GD10317]

General Information

Symbol

Dmel\Msp300^GD10317

Species

D. melanogaster

Name

FlyBase ID

FBal0207652

Feature type

allele

Associated gene

Dmel\Msp300

Associated Insertion(s)

Carried in Construct

P{GD10317}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10317}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10317

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^how-24B

(Morel et al., 2014)

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^how-24B

(Morel et al., 2014)

nucleus | third instar larval stage, with Scer\GAL4^how-24B

(Morel et al., 2014)

Detailed Description

Statement

Reference

Expression of Msp300^GD10317 under the control of Scer\GAL4^how-24B (muscle-specific) but not Scer\GAL4^elav.PU (neural) leads to larval locomotion deficit (reduced crawling ability - distance crawled/time) and also clustering of the muscle nuclei. The effect is further strengthened by addition of UAS-Dicer2 to enhance the RNAi efficiency.

(Morel et al., 2014)

Adults expressing Msp300^GD10317 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v50192

w¹¹¹⁸ P{GD10317}v50192

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Msp-300^GD10317

Msp300^GD10317

(Morton et al., 2025)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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