FlyBase Allele Report: Dmel\Pepck1[GD9451]

General Information

Symbol

Dmel\Pepck1^GD9451

Species

D. melanogaster

Name

FlyBase ID

FBal0208048

Feature type

allele

Associated gene

Dmel\Pepck1

Associated Insertion(s)

Carried in Construct

P{GD9451}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9451}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9451

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by brat^HMS01121

(Hussain et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^Lsp2.3.1

(Matsuoka et al., 2017)

increased cell death | adult stage | female, with Scer\GAL4^Lsp2.3.1

(Matsuoka et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

germarium, with Scer\GAL4^Lsp2.3.1

(Matsuoka et al., 2017)

leg, with Scer\GAL4^Dll-em212

(Córdoba et al., 2016)

segment of leg, with Scer\GAL4^Dll-em212

(Córdoba et al., 2016)

Detailed Description

Statement

Reference

Expression of Pepck^GD9451 under the control of Scer\GAL4^en-e16E in third instar wing disc posterior compartments does not result in an increase in cell death, as compared to controls.

(Hussain et al., 2017)

Expression of Pepck^GD9451 under the control of Scer\GAL4^Lsp2.3.1 (using tub-Gal80[ts] to limit expression to adulthood) leads to increase in cell death in early germline cysts in the germaria and their reduced size compared to controls (no such increase is observed in vitellogenic follicles and the proportion of dying vitellogenic stage egg chambers is even decreased) and while it has no significant effect on germline stem cell proliferation it negatively affects their maintenance.

(Matsuoka et al., 2017)

The expression of Pepck^GD9451 under the control of Scer\GAL4^Dll-em212, in combination with Dicer-2 expression for efficient RNAi, results in joint defects and reduced tarsal size, as compared to controls.

(Córdoba et al., 2016)

Adults expressing Pepck^GD9451 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9451

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Pepck1^GD9451, Scer\GAL4^en-e16E is an enhancer of increased cell death | third instar larval stage phenotype of BubR1^GD10778, Scer\GAL4^en-e16E

(Hussain et al., 2017)

NOT Enhancer of

Statement

Reference

Pepck1^GD9451, Scer\GAL4^da.PU is a non-enhancer of abnormal cell cycle phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

Suppressor of

Statement

Reference

Pepck1^GD9451, Scer\GAL4^da.PU is a suppressor of abnormal size phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

Pepck1^GD9451, Scer\GAL4^da.PU is a suppressor of neoplasia phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

NOT Suppressor of

Statement

Reference

Pepck1^GD9451, Scer\GAL4^da.PU is a non-suppressor of abnormal cell cycle phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

Other

Statement

Reference

Pepck1^GD9451, Scer\GAL4^en-e16E, mad2^GD16427 has increased cell death | third instar larval stage phenotype

(Hussain et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Pepck1^GD9451, Scer\GAL4^en-e16E is an enhancer of wing disc posterior compartment | third instar larval stage phenotype of BubR1^GD10778, Scer\GAL4^en-e16E

(Hussain et al., 2017)

Suppressor of

Statement

Reference

Pepck1^GD9451, Scer\GAL4^da.PU is a suppressor | partially of larval brain phenotype of Scer\GAL4^da.PU, brat^HMS01121

(Hussain et al., 2017)

Other

Statement

Reference

Pepck1^GD9451, Scer\GAL4^en-e16E, mad2^GD16427 has wing disc posterior compartment | third instar larval stage phenotype

(Hussain et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Posterior compartments of third instar wing discs expressing both Pepck^GD9451 and mad2^GD16427 under the control of Scer\GAL4^en-e16E exhibit a significant increase in cell death, as compared to controls.

Co-expression of Pepck^GD9451 enhances the increased cell death seen in wing disc posterior compartments expressing BubR1^GD10778 under the control of Scer\GAL4^en-e16E.

The larval brain overgrowth exhibited upon expression of brat^HMS01121 under the control of Scer\GAL4^da.PU is suppressed when Pepck^GD9451 is co-expressed. The proportion of defective anaphases and the level of cell death seen in larval brains expressing brat^HMS01121 under the control of Scer\GAL4^da.PU is not significantly changed upon co-expression of Pepck^GD9451. The tumor growth rate of larval brain explants expressing brat^HMS01121 under the control of Scer\GAL4^da.PU is suppressed upon co-expression of Pepck^GD9451.

(Hussain et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v20529

w¹¹¹⁸; P{GD9451}v20529

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9451

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pepck1^GD9451

Pepck^GD9451

(Matsuoka et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (12)

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