FlyBase Allele Report: Dmel\Pka-R2[GD15709]

General Information

Symbol

Dmel\Pka-R2^GD15709

Species

D. melanogaster

Name

FlyBase ID

FBal0208085

Feature type

allele

Associated gene

Dmel\Pka-R2

Associated Insertion(s)

Carried in Construct

P{GD15709}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD15709}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD15709

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Iijima-Ando et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing Pka-R2^GD15709 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

15709

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pka-R2^GD15709, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^elav-C155

(Iijima-Ando et al., 2009)

NOT Suppressor of

Statement

Reference

Pka-R2^GD15709, Scer\GAL4^elav-C155 is a non-suppressor of partially lethal - majority die phenotype of Hsap\HTT^{128Q.1-208.UAS}, Scer\GAL4^elav-C155

(Gonzales et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Pka-R2^GD15709 does not suppress lethality in flies with expression of Hsap\HTT^{128Q.1-208.Scer\UAS} driven by Scer\GAL4^elav-C155 (either the weak F27B or strong M64 insertion).

(Gonzales et al., 2016)

Co-expression of Pka-R2^GD15709 suppresses the locomotor defects in flies induced by Scer\GAL4^elav-C155-driven overexpression of Hsap\APP^{Aβ1-42.Scer\UAS.cIa}.

(Iijima-Ando et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v39437

w¹¹¹⁸; P{GD15709}v39437

Notes on Origin

Discoverer