FlyBase Allele Report: Dmel\Ras64B[GD1517]

General Information

Symbol

Dmel\Ras64B^GD1517

Species

D. melanogaster

Name

FlyBase ID

FBal0208239

Feature type

allele

Associated gene

Dmel\Ras64B

Associated Insertion(s)

Carried in Construct

P{GD1517}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1517}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1517

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Park et al., 2013)

ameliorates Huntington's disease

modeled by Hsap\HTT^NT.128Q.UAS

(Miller et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing Ras64B^GD1517 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1517

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ras64B^GD1517, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Park et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Ras64B^GD1517, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Park et al., 2013)

Ras64B^GD1517, Scer\GAL4^GMR.PF is a suppressor of photoreceptor phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Park et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Ras64B^GD1517 partially suppresses the external eye phenotype and photoreceptor degeneration seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

(Park et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v6225

w¹¹¹⁸; P{GD1517}v6225

Notes on Origin

Discoverer