FlyBase Allele Report: Dmel\ena[GD8910]

General Information

Symbol

Dmel\ena^GD8910

Species

D. melanogaster

Name

FlyBase ID

FBal0209516

Feature type

allele

Associated gene

Dmel\ena

Associated Insertion(s)

Carried in Construct

P{GD8910}

Also Known As

UAS-ena-RNAi, ena-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8910}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8910

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates chronic myeloid leukemia

modeled by Hsap\ABL1::Hsap\BCR^UAS.cBa

(Bernardoni et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | P-stage, with Scer\GAL4^kirre.PU

(Segal et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

dorsal-ventral compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^sd-SG29.1

(Becam et al., 2011)

filopodium | P-stage, with Scer\GAL4^kirre.PU

(Segal et al., 2016)

myotube | P-stage, with Scer\GAL4^kirre.PU

(Segal et al., 2016)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of ena^GD8910 under the control of Scer\GAL4^kirre.PU (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in myotubes with significantly shorter filopodia at 18-20 hrs APF, but filopodia density remains unchanged; and also leads to fusion defects in myotubes, with a reduction in the number of small nuclei originating from fusing myoblasts, and most myoblasts remain at a greater distance from the myotube; indirect flight muscles in these flies exhibit defects including muscle fiber splitting, as compared to wild type.

(Segal et al., 2016)

Late third instar mutant wing discs expressing ena^GD8910 under the control of Scer\GAL4^sd-SG29.1 exhibit increased boundary cell proliferation, compromising the dorsal-ventral affinity boundary. The anterior-posterior boundary is unaffected.

(Becam et al., 2011)

Adults expressing ena^GD8910 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8910

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^kirre.PU, ena^GD8910 has abnormal cell shape | P-stage phenotype, enhanceable by IRSp53^HMC02920, Scer\GAL4^kirre.PU

(Segal et al., 2016)

Suppressor of

Statement

Reference

ena^GD8910, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

ena^GD8910, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal size | adult stage phenotype of Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

ena^GD8910, Scer\GAL4^GMR.PU is a suppressor | partially of decreased cell number | third instar larval stage phenotype of Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^kirre.PU, ena^GD8910 has myotube | P-stage phenotype, enhanceable by IRSp53^HMC02920, Scer\GAL4^kirre.PU

(Segal et al., 2016)

Scer\GAL4^kirre.PU, ena^GD8910 has filopodium | P-stage phenotype, enhanceable by IRSp53^HMC02920, Scer\GAL4^kirre.PU

(Segal et al., 2016)

Suppressor of

Statement

Reference

ena^GD8910, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

ena^GD8910, Scer\GAL4^GMR.PU is a suppressor | partially of eye disc posterior to the morphogenetic furrow | third instar larval stage phenotype of Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of IRSp53^HMC02920 does not significantly enhance the effect on myotube filopodia length, but does result in a significant reduction in filopodia density, and an increase in severity of subsequent muscle fiber splitting in the indirect flight muscle of flies expressing ena^GD8910 under the control of Scer\GAL4^kirre.PU.

(Segal et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v43056