Expression via "HHLT-gal4" results in loss of progenitor cells in the primary lobes of the larval lymph gland. ("HHLT-gal4" is a combination of Scer\GAL4^HCH.Hand and Scer\GAL4^Hml.Δ that express in early lymph gland blood cells (hemocytes) and circulating and sessile blood cells, respectively, and incorporates Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} to maintain expression in the lymph gland after Scer\GAL4^HCH.Hand is down-regulated.)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increased number and size of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

Adults expressing lolal^GD4694 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of lolal^GD4694 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD4694} insertion line used.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies, adult/pharate lethality or early pupal lethality.