FlyBase Allele Report: Dmel\spn-A[GD5050]

General Information

Symbol

Dmel\spn-A^GD5050

Species

D. melanogaster

Name

FlyBase ID

FBal0210664

Feature type

allele

Associated gene

Dmel\spn-A

Associated Insertion(s)

Carried in Construct

P{GD5050}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5050}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5050

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing spn-A^GD5050 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5050

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, spn-A^GD5050 has abnormal DNA repair | larval stage | conditional phenotype

(Dekanty et al., 2015)

Phenotype Manifest In

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, spn-A^GD5050 has wing disc posterior compartment | conditional phenotype

(Dekanty et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The Scer\GAL4^en.PU-driven co-expression of BacA\p35^UAS.cHa and spn-A^GD5050 leads to a longer sustained DNA damage response to ionizing ration (assessed by phospho-H2Av immunostaining) in the wing disc posterior compartment as compared to the BacA\p35^UAS.cHa single expression or wild-type backgrounds.

(Dekanty et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v13362

w¹¹¹⁸ P{GD5050}v13362

v14021

w¹¹¹⁸; P{GD5050}v14021/TM3