Expression of zpg^GD2338 under the control of Scer\GAL4^ey-OK107 in adults does not significantly affect 3 hour aversive olfactory memory or the residual anaesthesia-resistant memory (ARM) after cold-shock, compared to controls; expression under the control of Scer\GAL4^VT000765 also does not affect ARM, compared to controls.

Expression of zpg^GD2338 under the control of Scer\GAL4^17d, but not the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R13F02, the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02, or the split-GAL4 pair of Scer\GAL4^DBD.R14C08 and Hsap\RELA^AD.R12C11, prevents the significant visual short term memory seen in controls following training with pain stimulus in adult flies; no memory defect is seen using Scer\GAL4^17d when expression is restricted to a smaller subset of cells using Scer\GAL80^Mef2.mb247.

Expression of zpg^GD2338 under the control of Scer\GAL4^Mef2.247 significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls; this effect is not seen when zpg^GD2338 is expressed during the adult stage only, under the control of Scer\GAL4^{Mef2.247.Switch} (induced by RU486 application for three days prior to the experiment).

Expression of zpg^GD2338 in both the anterior paired lateral (APL) and dorsal paired medial (DPM) neurons in the mushroom body (using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously, and in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) has no effect on 3 hour memory after associative conditioning.

Adults expressing zpg^GD2338 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.