FB2026_02 , released June 18, 2026
Allele: Dmel\OrcoUAS.YFP(2)
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General Information
Symbol
Dmel\OrcoUAS.YFP(2)
Species
D. melanogaster
Name
FlyBase ID
FBal0210972
Feature type
allele
Associated gene
Dmel\Orco
Associated Insertion(s)
Carried in Construct
P{UAS-Orco.YFP(2)}
Key Links
Transgenic product class
wild_type
(Benton et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Benton et al., 2006)
Mutagen
in vitro construct
(Benton et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Orco.YFP(2)}
Cytology
Description

UAS regulatory sequences drive expression of Orco tagged with YFP(2), a non-fluorescent split fluorescent protein fragment. Designed to be used in a protein fragment fluorescence complementation assay to study protein-protein interactions in vivo.

(Benton et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Orco
Tagged with
YFP(2)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Coexpression of Or43aScer\UAS.T:Avic\GFP-YFP(1) and Or83bScer\UAS.T:Avic\GFP-YFP(2), under the control of Scer\GAL4Gr21a.9.323, reconstitutes a functional odorant receptor as measured in neurons that also express the calcium-sensitive reporter Ggal\MLCK::Rat\CamG-CaMP.Scer\UAS.T:Avic\GFP-cpEGFP. Expression of Or43aScer\UAS.T:Avic\GFP-YFP(1) and Or83bScer\UAS.T:Avic\GFP-YFP(2) results in odor-evoked calcium responses to cyclohexanol and benzaldehyde across broad domains of the antennal lobe, while expression of Ggal\MLCK::Rat\CamG-CaMP.Scer\UAS.T:Avic\GFP-cpEGFP alone results in only sparse activation patterns.

      (Benton et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      This construct is designed for a "protein-fragment complementation assay (PCA)"; the T:Avic\GFP-YFP C-terminal fragment is complementary to an N-terminal T:Avic\GFP-YFP fragment which is carried on separate constructs. Binding of proteins tagged with either of the two T:Avic\GFP-YFP fragments brings them in close enough proximity to fold into an active form and fluoresce.

      (Benton et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Or83bScer\UAS.T:Avic\GFP-YFP(2)
      OrcoScer\UAS.T:Avic\GFP-YFP(2)
      OrcoUAS.YFP(2)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)