FlyBase Allele Report: Dmel\Rbfox1[CC00511]

General Information

Symbol

Dmel\Rbfox1^CC00511

Species

D. melanogaster

Name

FlyBase ID

FBal0211637

Feature type

allele

Associated gene

Dmel\Rbfox1

Associated Insertion(s)

P{PTT-GC}Rbfox1^CC00511

Carried in Construct

Also Known As

Rbfox1-GFP

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Buszczak et al., 2007)

Progenitor genotype

Associated Insertion(s)

P{PTT-GC}Rbfox1^CC00511

Cytology

Description

Insertion within an intron located between two coding exons, in the appropriate predicted reading frame to fuse the GFP exon in the inserted construct to the endogenous protein.

(Buszczak et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{PTT-GC}

protein trap

Tagged with

EGFP

green fluorescent protein

Tag:polyHis

epitope tag

purification tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:10,522,508..10,522,508 [+]

(Buszczak et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

female germline cyst

(Tastan et al., 2010)

follicle cell

Comment: germarium region 2b on

(Tastan et al., 2010)

oocyte

(Tastan et al., 2010)

germarium

(Tastan et al., 2010)

Additional Information

Statement

Reference

Expression is first observed in the cytoplasm of early germline cysts. In germarium region 3, expression shifts from the cytoplasmic to the nucleus of germline cells where it persists until the end of oogenesis. Expression is observed in follicle cells beginning in germarium region 2b.

(Tastan et al., 2010)

Marker for

Reflects expression of

Rbfox1

(Tastan et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile

(Tastan et al., 2010)

female fertile (with Rbfox1^f02600)

(Tastan et al., 2010)

viable

(Buszczak et al., 2007)

Phenotype Manifest In

nurse cell

(Tastan et al., 2010)

nurse cell (with Rbfox1^f02600)

(Tastan et al., 2010)

Detailed Description

Statement

Reference

Hemizygous and A2bp1^CC00511/A2bp1^f02600 females are fertile but a large fraction of their egg chambers contain 31 nurse cells and one oocyte. The extra nurse cells in A2bp1^CC00511 egg chambers arise from an extra round of mitosis prior to the egg chambers pinching off from the germarium.

(Tastan et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Rbfox1^CC00511 has egg chamber phenotype, enhanceable by enc^Q4

(Tastan et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51536

y¹ w^*; P{PTT-GC}Rbfox1^CC00511/TM3, Ser¹ Sb¹

Notes on Origin

Discoverer

Comments

Protein trap line.

(Buszczak et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

A2BP1^CC00511

(Tastan et al., 2010)

A2bp1^CC00511

CG32062^CC00511

Rbfox1^CC00511

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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