FlyBase Allele Report: Dmel\RpL10Ab[CB02653]

General Information

Symbol

Dmel\RpL10Ab^CB02653

Species

D. melanogaster

Name

FlyBase ID

FBal0211728

Feature type

allele

Associated gene

Dmel\RpL10Ab

Associated Insertion(s)

P{PTT-GB}RpL10Ab^CB02653

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Buszczak et al., 2007)

Progenitor genotype

Associated Insertion(s)

P{PTT-GB}RpL10Ab^CB02653

Cytology

Description

P{PTT-GB}RpL10Ab^CB02653 is inserted -34bp upstream of the RpL10Ab transcriptional start site.

(Wonglapsuwan et al., 2011)

Insertion upstream of the transcription start site (insertion is 1-500bp upstream of the start site), in the appropriate predicted reading frame to fuse the GFP exon in the inserted construct to the endogenous protein.

(Buszczak et al., 2007)

Allele components

Component

Use(s)

Inserted element

Tagged with

green fluorescent protein

Tag:polyHis

epitope tag

purification tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:11,822,818..11,822,818 [+]

(Wonglapsuwan et al., 2011, Buszczak et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval optic neuroblast

(Sen and Cox, 2016)

Additional Information

Statement

Reference

Marker for

Reflects expression of

RpL10Ab

(Sen and Cox, 2016)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Buszczak et al., 2007)

lethal - all die before end of larval stage

(Wonglapsuwan et al., 2011)

Phenotype Manifest In

egg chamber | germline clone

(Wonglapsuwan et al., 2011)

follicle cell | germline clone

(Wonglapsuwan et al., 2011)

nucleus | germline clone

(Wonglapsuwan et al., 2011)

Detailed Description

Statement

Reference

RpL10Ab^CB02653 germline clones fail to progress into mid-oogenesis. Approximately 36% of dying egg chambers exhibit a loss of follicle cells around the egg chamber, while the nurse cells appear normal. Nuclei of RpL10Ab^CB02653 germline follicle cells display abnormal morphology and are much larger than heterozygous nuclei. Moreover, the nucleoli of these clones are larger and more prominent compared to controls. RpL10Ab^CB02653 germline clones are larger and reduced in number compared to controls, suggesting a defect in proliferation or survival. Only 19% of RpL10Ab^CB02653 stage 10 egg chambers exhibit follicle cell clones.

(Wonglapsuwan et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RpL10Ab^CB02653 has lethal - all die before end of larval stage phenotype, suppressible | partially by Fmer\RpL10A^UAS.cWa/Scer\GAL4^Act5C.PI

(Wonglapsuwan et al., 2011)

RpL10Ab^CB02653 has lethal - all die before end of larval stage phenotype, suppressible | partially by Fmer\RpL10A^UAS.cWa/Scer\GAL4^arm.PS

(Wonglapsuwan et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Ubiquitous expression of Zzzz\RpL10A^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI, leads to 37% viability of RpL10Ab^CB02653 flies whereas expression under the control of Scer\GAL4^arm.PS leads to 39% viability in RpL10Ab^CB02653 mutants. In addition, these rescued flies are fertile, indicating that Zzzz\RpL10A^Scer\UAS.cWa has functional conservation with RpL10Ab.

(Wonglapsuwan et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Protein trap line.

(Buszczak et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RpL10Ab^CB02653

(Voo et al., 2021, Sen and Cox, 2016, Wonglapsuwan et al., 2011)

RpL10Ab^CB2653

(Voo et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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