Females containing germline clones of mamo^SVA53 rarely exhibit differentiating egg chambers and mature eggs when compared to controls.

Homozygous mamo^SVA53 germline cells exhibit chromatin condensation defects, with approximately 45.5% of nuclei exhibit less-condensed chromatin, compared to 99% of wild-type germline cells exhibiting highly condensed chromatin.

mamo^SVA53 embryos derived from mamo^SVA53/mamo^SVA53 germline clones fertilized by wild-type sperm form normal numbers of pole cells, most of which successfully migrate into the embryonic gonads.

Embryos resulting from the transplantation of mamo^SVA53 pole cells into host embryos that lack pole cells develop into sterile flies. Although females transplanted with mamo^SVA53 pole cells are able to lay eggs, these eggs fail to initiate cleavage division and therefore do not develop. In oocytes drived from mamo^SVA53 pole cells, initiation of meiosis is not affected but the disassembly of the synaptonemal complex is retarded. Karyosomes are severely fragmented in 32% of mamo^SVA53 oocytes during stages 8-10. After ovulation, meiotic divisions are severely impaired and most of the meiotic divisions are scattered. Meiotic products, such as polar-body nuclei or rossette structures, are observed in 6.9% of mamo^SVA53 eggs. mamo^SVA53 eggs are capable of accepting sperm, however sperm nuclei fail to decondense and remain separated from female chromosomes until at least 1 hour AEL.

All host males transplanted with mamo^SVA53 pole cells are sterile.