FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\Mms19EY00797
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General Information
Symbol
Dmel\Mms19EY00797
Species
D. melanogaster
Name
FlyBase ID
FBal0217091
Feature type
allele
Associated gene
Dmel\Mms19
Associated Insertion(s)
P{EPgy2}Mms19EY00797
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{EPgy2}Mms19EY00797
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    P{EPgy2}
    Regulatory region(s)
    UASp
    Mutations Mapped to the Genome
    Curation Data
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Mms19EY00797 mutant larvae develop slowly but reach third instar stage, however the third instar larvae have noticeably smaller brains than wild-type, completely lack recognizable imaginal discs and fail to pupate.

        24.5% of embryos from mothers carrying Mms19EY00797 mutant germline clones (created using the OvoD technique) hatch into larvae if fertilized by Mms19+/+ fathers and 10.5% if fertilized by Mms19EY00797/+ fathers. Majority of the embryos display various cell cycle defects: spindle defects, kinked axis of division, many missing nuclei, elongated spindle in metaphase, chromosome segregation defects, and centrosomal defects in division cycle 10-13.

        Embryos from Mms19EY00797 mutant mothers also carrying Mms19EGFP (to rescue the maternal effect cell cycle defects) and expressing Zzzz\vhhGFP4deGradFP.hb.bcd3'UTR to knock-down the rescue Mms19EY00797 transgene during embryogenesis only, leads to various and abundant cell cycle defects: Improper chromosome segregation and chromosomal bridges in anaphase and telophase, significantly elongated spindle in metaphase, spindle crossovers, multipolar spindles, kinked axis of division and loss of nuclei in one or more large areas.

        (Nag et al., 2018)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Suppressed by
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        The various maternal effect cell cycle defects observed in early embryos from from mothers carrying Mms19EY00797 mutant germline clones (created using the OvoD technique) are partially suppressed by combination with a single copy of Xpdunspecified loss-of-function or a deficiency covering the Xpd locus (apart from the chromosome segregation defects, the frequency of which is actually increased).

        The complete loss of imaginal discs characteristic for Mms19EY00797 mutant third instar larvae can be rescued by simultaneous expression of Cdk7UAS.cNa, Mat1UAS.cNa and CycHUAS.cNa under the control of Scer\GAL4da.G32 in the mutant background, but the overall structure of the discs is visibly abnormal.

        (Nag et al., 2018)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        Mms19EY00797/Df(3R)ED5147 is rescued by Mms19EGFP

        (Nag et al., 2018)
        Comments

        Combination with one or two copies of Mms19EGFP rescues the lethality of Mms19EY00797/Df(3R)ED5147 mutants. Expression of Mms19EGFP in Mms19EY00797 germline mutant females (created using the OvoD technique) at least partially rescues all of the various cell cycle defects (spindle defects, kinked axis of division, many missing nuclei, elongated spindle in metaphase, chromosome segregation defects, and centrosomal defects) observed in their progeny at embryonic division cycle 10-13.

        (Nag et al., 2018)
        Images (0)
        Mutant
        Wild-type
        Stocks (2)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        Mms19EY00797
        Mms19P
        (Nag et al., 2018)
        Name Synonyms
        Secondary FlyBase IDs
          References (2)