FlyBase Allele Report: Dmel\gwl[6a]

General Information

Symbol

Dmel\gwl^6a

Species

D. melanogaster

Name

FlyBase ID

FBal0218853

Feature type

allele

Associated gene

Dmel\gwl

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Archambault et al., 2007)

Mutagen

P-element activity

(Archambault et al., 2007)

Progenitor genotype

gwl^EP515

(Archambault et al., 2007)

Cytology

Description

Imprecise excision of P{EP}gwl^EP515 removes the N-terminal end of gwl and it's 5' upstream region.

(Archambault et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion rhythm (with gwl^Sr3)

(Archambault et al., 2007)

abnormal mitotic cell cycle (with Df(3L)gwl^3b)

(Archambault et al., 2007)

abnormal mitotic cell cycle (with gwl^Sr3)

(Archambault et al., 2007)

abnormal mitotic cell cycle | dominant | embryonic stage | maternal effect

(Wang et al., 2013)

female fertile (with gwl^Sr3)

(Archambault et al., 2007)

female fertile (with gwl^Sr6)

(Archambault et al., 2007)

female sterile (with gwl^Sr18)

(Wang et al., 2013)

fertile (with gwl^Sr6)

(Archambault et al., 2007)

partially lethal - majority die

(Archambault et al., 2007)

partially lethal - majority live (with gwl^Sr3)

(Archambault et al., 2007)

sterile

(Archambault et al., 2007)

sterile (with gwl^Sr3)

(Archambault et al., 2007)

Phenotype Manifest In

chromatin (with Df(3L)gwl^3b)

(Archambault et al., 2007)

chromatin (with gwl^Sr3)

(Archambault et al., 2007)

eye (with gwl^Sr3)

(Archambault et al., 2007)

gonad (with gwl^Sr3)

(Archambault et al., 2007)

macrochaeta (with gwl^Sr3)

(Archambault et al., 2007)

wing margin (with gwl^Sr3)

(Archambault et al., 2007)

Detailed Description

Statement

Reference

Embryos hatch at a normal rate from gwl^6a/+ mothers.

Syncitial embryos from gwl^6a/+ mothers show a low frequency of nuclei that fail to enter mitosis when the majority of nuclei are already in pro/metaphase.

(Wang et al., 2013)

gwl^6a mutants are sterile in both males and females.

gwl^6a/gwl^Sr3 mutants produce a gwl^Sr3 phenotype, i.e female fertility is restored.

gwl^6a/gwl^Sr6 mutants produce a gwl^Sr3 phenotype, i.e female fertility is restored.

A significant proportion of gwl^6a/Df(3L)gwl^3b cells exhibit polyploid mitotic figures. They also exhibit significant pre-anaphase delay with hyper-condensed chromosomes.

(Archambault et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

gwl^6a has abnormal mitotic cell cycle | embryonic stage | maternal effect | dominant phenotype, enhanceable by polo^UASp.Tag:MYC/Scer\GAL4^{VP16.mat.αTub67C}

(Wang et al., 2013)

Enhancer of

Statement

Reference

gwl[+]/gwl^6a is an enhancer of partially lethal | maternal effect phenotype of Scer\GAL4^{VP16.mat.αTub67C}, polo^UASp.Tag:MYC

(Wang et al., 2013)

gwl[+]/gwl^6a is an enhancer of some die during embryonic stage | maternal effect phenotype of Scer\GAL4^{VP16.mat.αTub67C}, polo^UASp.Tag:MYC

(Wang et al., 2013)

gwl[+]/gwl^6a is an enhancer of lethal | maternal effect phenotype of Scer\GAL4^{VP16.mat.αTub67C}, polo^{T182D.UASp.Tag:MYC}

(Wang et al., 2013)

Suppressor of

Statement

Reference

gwl[+]/gwl^6a is a suppressor of partially lethal - majority die | dominant | maternal effect | embryonic stage phenotype of mts^XE-2258/mts[+], polo¹¹

(Wang et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}-mediated expression of polo^{Scer\UAS.P\T.T:Hsap\MYC} in gwl^6a/+ mothers results in a marked decrease in embryo hatching rate compared to either single genotype.

Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}-mediated expression of polo^{Scer\UAS.P\T.T:Hsap\MYC} in embryos from gwl^6a/+ mothers show a significant fraction of nuclei that fail to enter mitosis when the majority of nuclei are already in pro/metaphase. Defective nuclei are characterized by absence of a mitotic spindle, very strong lamin staining, and uncondensed chromosomes.

(Wang et al., 2013)

gwl^6a/+ partially rescues the viability of embryos from mts^XE-2258/+, polo¹¹/+ mothers.

(Wang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

gwl^6a/gwl^Sr18 is partially rescued by gwl^UASp.Tag:MYC/Scer\GAL4^{VP16.mat.αTub67C}

(Wang et al., 2013)

Not rescued by

gwl^6a/gwl^Sr18 is not rescued by gwl^{NLSmut.UASp.Tag:MYC}/Scer\GAL4^{VP16.mat.αTub67C}