FlyBase Allele Report: Dmel\mbt[S482N.S521E.UAS]

General Information

Symbol

Dmel\mbt^{S482N.S521E.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0218873

Feature type

allele

Associated gene

Dmel\mbt

Associated Insertion(s)

Carried in Construct

P{UAS-mbt.S482N.S521E}

Key Links

Transgenic product class

missense_variant

(Menzel et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Menzel et al., 2007)

Mutagen

in vitro construct

(Menzel et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-mbt.S482N.S521E}

Cytology

Description

UASt sequences govern expression of the mutated mbt ORF (carries the amino acid replacements S482N and S521E).

(Menzel et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mbt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

Phenotype Manifest In

cone cell | pupal stage P7, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

eye, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

eye photoreceptor cell, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

filamentous actin & eye photoreceptor cell | third instar larval stage, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

filamentous actin & ommatidium | pupal stage P7, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

interommatidial bristle | pupal stage P7, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

nucleus & eye photoreceptor cell | third instar larval stage, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

ommatidial precursor cluster | third instar larval stage, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

ommatidium, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

ommatidium | pupal stage P7, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

pigment cell & retina | pupal stage P7, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

rhabdomere & eye photoreceptor cell, with Scer\GAL4^elav.PLu

(Menzel et al., 2007)

Detailed Description

Statement

Reference

mbt^{S482N.S521E.Scer\UAS}; Scer\GAL4^elav.PLu flies have a severe rough eye phenotype. Most ommatidia contain fewer photoreceptor cells and rhabdomeres are misshapen. Depending on the insertion of P{UAS-mbt.S482N.S521E}, eye size my be reduced and some parts of the eye appear glazed. At the late third instar larval stage, eye discs in these animals have only mild phenotypes: filamentous actin distribution is irregular and the spacing of developing ommatidial clusters is disturbed and the nuclei of developing photoreceptors fail to remain apical. By the mid-pupal stage, ommatidial structure is completely disturbed: The arrangement of F-actin and the numbers and arrangement of cone cells bristle cells and pigment cells are severely disrupted.

mbt^{S482N.S521E.Scer\UAS}; Scer\GAL4^GMR.PF flies have small, glazed eyes with no discernable ommatidial structure.

(Menzel et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mbt^{S482N.S521E.Scer\UAS}

mbt^{S482N.S521E.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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