FB2026_02 , released June 18, 2026
Allele: Dmel\Pkc53E::Pknhs.PB
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General Information
Symbol
Dmel\Pkc53E::Pknhs.PB
Species
D. melanogaster
Name
heat shock construct of Betson
FlyBase ID
FBal0219006
Feature type
allele
Associated gene
Pkc53E::Pkn
Associated Insertion(s)
Carried in Construct
P{hs-Pkn-PKC53E}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Betson and Settleman, 2007)
Progenitor genotype
Carried in construct
P{hs-Pkn-PKC53E}
Cytology
Description

A heat shock promoter drives expression of a chimeric protein in which the kinase domain of Pkn has been replaced with the kinase domain of Pkc53E.

(Betson and Settleman, 2007)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Pkc53E::Pkn
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Pkc53E::Pknhs.PB using a heat shock regime of 30 minutes once a day causes substantial lethality.

      (Betson and Settleman, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Pkc53E::Pknhs.PB is a suppressor of lethal phenotype of Pkn06736

      (Betson and Settleman, 2007)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Pkc53E::Pknhs.PB (using a reduced heat shock regime of 15 minutes per day) can partially rescue the lethality of Pkn06736.

      (Betson and Settleman, 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pkc53E::Pknhs.PB
      Name Synonyms
      heat shock construct of Betson
      Secondary FlyBase IDs
        References (1)