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General Information
Symbol
Dmel\Ir84aUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Benton
FlyBase ID
FBal0220387
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-IR84a
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the full length Ir84a open reading frame.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Ir84aScer\UAS.cBa under the control of Scer\GAL4Or35a.PF results in a strong olfactory response to phenylacetaldehyde in the ac3 sensilla (a response not seen in the wild-type ac3 sensilla), although a response to phenylacetonitrile is not seen in the ac3 sensilla in these flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.

Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aΔATD.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 fail to respond to phenylacetaldehyde.

Neurons co-expressing Ir8aΔC.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP and Ir8aScer\UAS.cAa under the control of Scer\GAL4Or22a.7.717 fail to respond to phenylacetaldehyde.

Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aR481A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.

Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aR481E.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 show a moderate but significant reduction in phenylacetaldehyde responses.

Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aT645A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.

Neurons co-expressing Ir84aScer\UAS.cBa and Ir8aD724A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP under the control of Scer\GAL4Or22a.7.717 fail to respond to phenylacetaldehyde.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Ir84aScer\UAS.cBa using Scer\GAL4Ir84a-GAL4 restores the response to phenylacetic acid and phenylacetaldehyde in Ir84aGAL4 homozygotes. The reduced courtship seen in Ir84aGAL4 males is also rescued.

The increased courtship seen in wild type males when killed female objects are perfumed with phenylacetic acid (or fly food) is not observed in Ir84aGAL4 males, but this is partially restored by expression of Ir84aScer\UAS.cBa.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Generated using the phiC31-based integration system.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Ir84aScer\UAS.cBa
Ir84aUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Benton
Secondary FlyBase IDs
    References (5)