FlyBase Allele Report: Dmel\Vps15[5]

General Information

Symbol

Dmel\Vps15⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0220468

Feature type

allele

Associated gene

Dmel\Vps15

Associated Insertion(s)

Carried in Construct

Also Known As

ird1⁵

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wu et al., 2007)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Wu et al., 2007)

Amino acid replacement: Q144term.

(Wu et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,245,070..9,245,070 [-]

Nucleotide change:

C9245070T

Amino acid change:

Q144term | Vps15-PA; Q144term | Vps15-PB; Q144term | Vps15-PC

Reported amino acid change:

Q144term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wu et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | somatic clone

(O'Farrell et al., 2017)

abnormal immune response (with Vps15¹)

(Wu et al., 2007)

abnormal neuroanatomy | adult stage | somatic clone

(Issman-Zecharya and Schuldiner, 2014)

decreased cell growth rate (with Vps15¹)

(Powis and Macdougall, 2011)

lethal (with Df(3R)Δvps15)

(Lindmo et al., 2008)

Phenotype Manifest In

embryonic/larval fat body (with Vps15¹)

(Powis and Macdougall, 2011)

eye-antennal disc | larval stage | somatic clone

(O'Farrell et al., 2017)

follicle cell | somatic clone

(O'Farrell et al., 2017)

gamma Kenyon cell | adult stage | somatic clone

(Issman-Zecharya and Schuldiner, 2014)

imaginal disc epithelial cell | somatic clone

(O'Farrell et al., 2017)

Detailed Description

Statement

Reference

Vps15⁵ somatic clones in the follicular epithelium exhibit dysplasia-like defects, namely a high frequency of epithelium multilayering and mild cell polarity defects (i.e. abnormal expression of the adherent junction components Arm and E-cad, and occasional overlap between the apical marker aPKC and the basolateral marker Dlg), as compared to controls.

Vps15⁵ somatic clones also disrupt the larval eye-antennal epithelium, as compared to controls.

(O'Farrell et al., 2017)

Homozygous adult mushroom body gamma neuron clones show an axon pruning defect.

(Issman-Zecharya and Schuldiner, 2014)

ird1¹/ird1⁵ mutant fat body cells are smaller than in wild-type controls at 72 hours after egg laying. However, these cells appear the same size as in controls by 96 hours after egg laying.

(Powis and Macdougall, 2011)

ird1¹/ird1⁵ flies show significantly reduced survival compared to control flies after injection with either E.coli or M.luteus.

(Wu et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Vps15⁵ has neoplasia | somatic clone | larval stage phenotype, suppressible | somatic clone by Lkb1^X5

(O'Farrell et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Vps15⁵ has abnormal developmental rate phenotype, suppressible | somatic clone by Lkb1^X5

(O'Farrell et al., 2017)

Vps15⁵ has abnormal cell polarity | somatic clone phenotype, suppressible by Lkb1^X5

(O'Farrell et al., 2017)

Enhancer of

Statement

Reference

Vps15⁵ is an enhancer of neoplasia | larval stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(O'Farrell et al., 2017)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Vps15⁵ has abnormal developmental rate phenotype

(O'Farrell et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Vps15⁵ has eye-antennal disc | somatic clone | larval stage phenotype, suppressible | somatic clone by Lkb1^X5

(O'Farrell et al., 2017)

Vps15⁵ has follicle cell | somatic clone phenotype, suppressible by Lkb1^X5

(O'Farrell et al., 2017)

Enhancer of

Statement

Reference

Vps15⁵ is an enhancer of eye-antennal disc | larval stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(O'Farrell et al., 2017)

NOT Enhancer of

Statement

Reference

Vps15⁵ is a non-enhancer of follicle cell | somatic clone phenotype of Lkb1^X5

(O'Farrell et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Vps15⁵, Lkb1^X5 double mutant somatic clones in the follicular epithelium exhibit multilayering at a frequency similar to that of Lkb1^X5 single clones rather than the high frequency observed in Vps15⁵ single clones. The mis-localization of cell polarity markers presented by Vps15⁵ homozygous follicle cell clones is also suppressed by Lkb1^X5 homozygosity in the clones.

The induction of larval eye-antennal disc clones both homozygous for Vps15⁵ and expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI leads to an enhanced eye-antennal disc tumor phenotype, as compared to clones only expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI, and leads to delayed pupation, as compared to controls. Both these phenotypes are suppressed by additional Lkb1^X5 homozygosity in the clones.

(O'Farrell et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Vps15⁵

ird1⁵

(Powis and Macdougall, 2011, Lindmo et al., 2008, Wu et al., 2007)

vps15⁵

(Issman-Zecharya and Schuldiner, 2014)

vps15^ird1-5

(O'Farrell et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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