FlyBase Allele Report: Dmel\bi[RNAi.UAS]

General Information

Symbol

Dmel\bi^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0220997

Feature type

allele

Associated gene

Dmel\bi

Associated Insertion(s)

Carried in Construct

P{UAS-bi.RNAi}

Also Known As

UAS-ombRNAi

Key Links

Transgenic product class

RNAi_reagent

(Shen et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Shen et al., 2008)

Mutagen

in vitro construct

(Shen et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-bi.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat corresponding to nucleotides 1363-1916bp of the bi coding sequence (covering the T-domain).

(Shen et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased size | adult stage, with Scer\GAL4^bi-md653

(Tsai et al., 2015)

increased size | third instar larval stage, with Scer\GAL4^bi-md653

(Tsai et al., 2015)

visible | adult stage, with Scer\GAL4^bi-md653

(Tsai et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^bi-md653

(Tsai et al., 2015)

eye disc | third instar larval stage, with Scer\GAL4^bi-md653

(Tsai et al., 2015)

wing blade, with Scer\GAL4^en-e16E

(Shen et al., 2008)

wing pouch, with Scer\GAL4^en-e16E

(Shen et al., 2008)

wing pouch, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Shen et al., 2008)

wing sensillum | ectopic, with Scer\GAL4^dpp.blk1

(Shen et al., 2008)

Detailed Description

Statement

Reference

Down-regulation of bi in the posterior compartment of the A/P boundary through the expression of bi^{dsRNA.Scer\UAS} by Scer\GAL4^en-e16E or Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} leads to strong apical fold formation along the A/P boundary of the larval wing pouch. At the end of ontogenesis, a big gap serpates A and P compartments in adult wings.

In contrast, anterior reduction of bi through expression of bi^{dsRNA.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 does not cause aberrant folding of the larval wing pouch and does not cause gap formation in the adult wing.

Clonal analysis reveals that posterior reduction of bi is sufficient for A/P fold formation. Posterior clones in which bi is reduced by bi^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^en-e16E) form an apical fold where the clone runs along the A/P boundary.

(Shen et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

bi^RNAi.UAS/Scer\GAL4^dpp.blk1 is a suppressor | partially of decreased size | third instar larval stage phenotype of Scer\GAL4^dpp.blk1, arm^UAS.cUa

(Tsai et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

bi^RNAi.UAS/Scer\GAL4^dpp.blk1 is a suppressor | partially of eye disc | third instar larval stage phenotype of Scer\GAL4^dpp.blk1, arm^UAS.cUa

(Tsai et al., 2015)

bi^RNAi.UAS/Scer\GAL4^dpp.blk1 is a suppressor | partially of eye phenotype of Scer\GAL4^dpp.blk1, arm^UAS.cUa

(Tsai et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

bi^RNAi.UAS

bi^{dsRNA.Scer\UAS}

bi^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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