FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\His3SL.HDE.EGFP
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General Information
Symbol
Dmel\His3SL.HDE.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0221473
Feature type
allele
Associated gene
His3
Associated Insertion(s)
Carried in Construct
P{His3-SL-HDE-EGFP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wagner et al., 2007)
Progenitor genotype
Carried in construct
P{His3-SL-HDE-EGFP}
Cytology
Description

The His3 promoter, amino acids 1-62 of the His3 open reading frame (ORF) and the His3 3' end containing a portion of the downstream intergenic region (from immediately after the stop codon through until 18bp downstream of the HDE) are fused upstream of the EGFP ORF. The His3 ORF is in frame with the EGFP ORF. A single mutation (U to A) has been introduced within the HDE in order to disrupt a stop codon.

(Wagner et al., 2007)

The EGFP open reading frame (ORF) and a poly(A) site is fused downstream of a fragment consisting of the His3 promoter and the first 62 amino acids of the His3 ORF fused to the 3'UTR of His3 (which includes the stem loop and histone downstream element (HDE) sequences but not the downstream polyadenylation signals).

(Wagner et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
His3
Encoded product / tool
His3
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      P{His3-SL-HDE-EGFP} acts as a reporter of histone pre-mRNA misprocessing; normal histone pre-mRNA processing results in mRNA lacking the Avic\GFP open reading frame, while misprocessing leads to readthrough of transcription and production of mRNA encoding Avic\GFP.

      (Wagner et al., 2007)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      His3SL.HDE.EGFP
      His3SL.HDE.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)