FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: P{His3-SL-HDE-EGFP}
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General Information
Symbol
P{His3-SL-HDE-EGFP}
FlyBase ID
FBtp0041071
Feature type
transgenic_transposable_element
Component Allele(s)
His3SL.HDE.EGFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele His3SL.HDE.EGFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
His3
Encoded product / tool
His3
Tagged with
EGFP
Description

The EGFP open reading frame (ORF) and a poly(A) site is fused downstream of a fragment consisting of the His3 promoter and the first 62 amino acids of the His3 ORF fused to the 3'UTR of His3 (which includes the stem loop and histone downstream element (HDE) sequences but not the downstream polyadenylation signals). The His3 ORF is in frame with the EGFP ORF. A single mutation (U to A) has been introduced within the HDE in order to disrupt a stop codon. The transgene acts as a reporter of histone pre-mRNA misprocessing; normal histone pre-mRNA processing results in mRNA lacking the EGFP open reading frame, while misprocessing leads to readthrough of transcription and production of mRNA encoding EGFP.

(Wagner et al., 2007)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P<up>GFP reporter</up>
(Wagner et al., 2007)
P{His3-SL-HDE-EGFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (4)