FlyBase Allele Report: Dmel\Atg13[Δ74]

General Information

Symbol

Dmel\Atg13^Δ74

Species

D. melanogaster

Name

FlyBase ID

FBal0221507

Feature type

allele

Associated gene

Dmel\Atg13

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chang and Neufeld, 2009)

Mutagen

P-element activity

(Chang and Neufeld, 2009)

Progenitor genotype

P{GSV6}Atg13^GS11822

(Chang and Neufeld, 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes 246bp flanking the annotated Atg13 transcription start site.

(Chang and Neufeld, 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | germline clone (with Atg13^Δ81)

(Barth et al., 2011)

fertile | germline clone (with Atg13^Δ81)

(Barth et al., 2011)

lethal - all die before end of pupal stage | recessive

(Chang and Neufeld, 2009)

some die during pupal stage | recessive

(Chang and Neufeld, 2009)

Phenotype Manifest In

embryonic/larval fat body | somatic clone

(Chang and Neufeld, 2009)

Detailed Description

Statement

Reference

Atg13^Δ74/Atg13^Δ81 mutant mosaic germline cells are defective in autophagy (as monitored by the lack of lysotracker staining). However, the chimeras are fully fertile with normal egg-laying behavior and hatching rates. There are no defects in egg chamber development or egg morphology. These germline mosaics do not show disruption of nurse cell apoptosis.

(Barth et al., 2011)

Homozygous clones in the larval fat body fail to induce autophagy in response to starvation.

(Chang and Neufeld, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Atg13^Δ74/Atg13^Δ74 is a non-enhancer of increased cell number | pupal stage phenotype of Df(2R)BSC696/hrm^Δ1

(Velentzas et al., 2018)

NOT Suppressor of

Statement

Reference

Atg13^Δ74/Atg13^Δ74 is a non-suppressor of increased cell number | pupal stage phenotype of Df(2R)BSC696/hrm^Δ1

(Velentzas et al., 2018)

Other

Statement

Reference

Atg13^Δ74, Scer\GAL4^fkh.PH, hrm^Δ1/hrm[+] has increased cell number | pupal stage phenotype

(Velentzas et al., 2018)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Atg13^Δ74/Atg13^Δ74 is a non-enhancer of salivary gland | pupal stage phenotype of Df(2R)BSC696/hrm^Δ1

(Velentzas et al., 2018)

NOT Suppressor of

Statement

Reference

Atg13^Δ74/Atg13^Δ74 is a non-suppressor of salivary gland | pupal stage phenotype of Df(2R)BSC696/hrm^Δ1

(Velentzas et al., 2018)

Other

Statement

Reference

Atg13^Δ74, Scer\GAL4^fkh.PH, hrm^Δ1/hrm[+] has salivary gland | pupal stage phenotype

(Velentzas et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The salivary gland tissue persistence phenotype observed in hrm^Δ1/Df(2R)BSC696 transheterozygous pupae is not affected by additional Atg13^Δ74 homozygosity; a similar salivary gland tissue persistence phenotype is observed in hrm^Δ1/+; Atg13^Δ74/Atg13^Δ74 individuals.

(Velentzas et al., 2018)

Autophagy is no longer induced by expression of Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Cg.PA in clones of cells that are also homozygous for Atg13^Δ74.

(Chang and Neufeld, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ATG13^Δ74

(Barth et al., 2012)

Atg13^D74

(Nandy et al., 2018)

Atg13^Δ74

(Velentzas et al., 2018, Lin et al., 2017, Chang et al., 2013, Barth et al., 2011)

Δ74

(Chang and Neufeld, 2009)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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