FlyBase Allele Report: Dmel\Cds[CB-0128-3]

General Information

Symbol

Dmel\Cds^CB-0128-3

Species

D. melanogaster

Name

FlyBase ID

FBal0223841

Feature type

allele

Associated gene

Dmel\Cds

Associated Insertion(s)

P{RS3}Cds^CB-0128-3

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Laurinyecz et al., 2016)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{RS3}Cds^CB-0128-3

Cytology

Description

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,128,118..8,128,118 [+]

(Laurinyecz et al., 2016, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile

(Laurinyecz et al., 2016)

male sterile (with Df(3L)BSC795)

(Laurinyecz et al., 2016)

Phenotype Manifest In

major mitochondrial derivative

(Laurinyecz et al., 2016)

minor mitochondrial derivative

(Laurinyecz et al., 2016)

spermatid

(Laurinyecz et al., 2016)

spermatid axoneme

(Laurinyecz et al., 2016)

spermatid cyst

(Laurinyecz et al., 2016)

waste bag

(Laurinyecz et al., 2016)

Detailed Description

Statement

Reference

CdsA^CB-0128-3 homozygous male adults as well as CdsA^CB-0128-3/Df(3L)BSC795 are completely sterile but CdsA¹/CdsA^CB-0128-3 males are fertile.

Adult CdsA^CB-0128-3 homozygote males have no mature sperm in the seminal vesicles and display spermatid individualization defects: the actin-rich investment cones are established but are disorganized and move asynchronously along the spermatids and neither a cystic bulge nor a proper waste bag is observed.

Adult CdsA^CB-0128-3 homozygote males do not display any defects in the morphology of mitochondria in primary spermatocytes, early round spermatids or elongating spermatids: the mitochondrial fusion, nebenkern formation as well as elongation is normal during these stages. However, elongated pre-individualized CdsA^CB-0128-3 cysts contain disorganized spermatids with either same-sized mitochondrial derivatives or enlarged, different-sized derivatives and also show overgrowth of the axonemal sheath.

Adult CdsA^CB-0128-3 homozygote males do not display normal climbing and flight abilities compared to wild-type.

(Laurinyecz et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cds^CB-0128-3 has male sterile phenotype, suppressible | partially by Pis^βTub85D.PL

(Laurinyecz et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cds^CB-0128-3 has spermatid phenotype, suppressible | partially by Pis^βTub85D.PL

(Laurinyecz et al., 2016)

Cds^CB-0128-3 has waste bag phenotype, suppressible | partially by Pis^βTub85D.PL

(Laurinyecz et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The adult male sterility characteristic for CdsA^CB-0128-3 homozygotes is partially rescued by expression of Pis^βTub85D.PL in the mutant background (the number of dispersed actin cones in the elongated spermatids is reduced and the number of waste bags is increased compared to CdsA^CB-0128-3 mutants).

(Laurinyecz et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cds^CB-0128-3 is rescued by Cds^UASp.EGFP

(Laurinyecz et al., 2016)

Comments

Expression of CdsA^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{bam.T:Hsim\VP16} rescues the spermatid individualization defects and adult male sterility of CdsA^CB-0128-3 homozygotes.

(Laurinyecz et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the P{RS3} element reverts the male sterile phenotype.

(Laurinyecz et al., 2016)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cds^CB-0128-3

CdsA^CB-0128-3

(Laurinyecz et al., 2016)

CdsA^ms1

(Laurinyecz et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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