UASt regulatory sequences drive expression of an EcoRI-NotI fragment encoding amino acids 1-347 of the ninaE product. A linker sequence encoding 3 glycine residues is included upstream of the NotI site. The EGFP sequence, flanked by NotI and XbaI sites, is inserted downstream of the ninaE and linker sequences.
eye, with Scer\GAL4GMR.PF
Flies expressing ninaEScer\UAS.CC.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PF develop relatively small eyes.