Expression of Pmm2^KK100043 under the control of Scer\GAL4^da.G32 leads to lethality in late larval stages or early P-stage, and animals also exhibit severe developmental delay. Third instar larvae display a significant reduction in coordinated movement compared to controls, as measured by rate of peristaltic waves; third instar muscle 4 neuromuscular junctions display over-elaboration, with more branches, greater synaptic terminal area, and supernumerary type Is/Ib synaptic boutons; neurotransmission strength is significantly increased in a two-electrode voltage clamp recording from third instar muscle 6, as compared to controls.

Expression of Pmm2^KK100043 under the control of Scer\GAL4^how-24B leads to reduced adult lifespan, but no ataxia, as compared to controls.

Expression of Pmm2^KK100043 under the control of Scer\GAL4^elav.PU leads to increased adult lifespan, as compared to controls.