Adulthood-only expression of Atg13^KK100340 under the combined control of Scer\GAL4^tub.PU and Gal80[ts] leads to intestinal barrier disfunction (as shown by the abnormal permeability to a non-absorbable food dye), leads to the failure to induce autophagy (assessed by the lysosome and autophagosome marker, Lysotracker) in the adult fat body in response to starvation, and leads to a decreased lifespan, as compared to controls. Adulthood-only expression under the combined control of Scer\GAL4^{Myo31DF-NP0001} and Gal80[ts] leads to pronounced defects in the midgut, namely a severe increase in cell size and abnormal epithelium morphology, as compared to controls.