FB2026_02 , released June 18, 2026
Allele: Dmel\wmdKK100974
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General Information
Symbol
Dmel\wmdKK100974
Species
D. melanogaster
Name
FlyBase ID
FBal0231018
Feature type
allele
Associated gene
Dmel\wmd
Associated Insertion(s)
Carried in Construct
P{KK100974}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK100974}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK100974
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of wmdKK100974 under the control of either Scer\GAL4Mef2.PR or Scer\GAL4elav.PU (both with Dcr-2Scer\UAS.cUa) or driven by any of the following: Scer\GAL4c179, Scer\GAL4how-24B or Scer\GAL4da.PU (all without Dcr-2Scer\UAS.cUa) reduces adult viability to a varying degree (up to a point of complete lethality in some cases) at both 25[o]C and 29[o]C with more severe effects being observed at the higher temperature. Expression under Scer\GAL4tub.PU is completely lethal at both temperatures while expression under either Scer\GAL4G7 or Scer\GAL4RapGAP1-OK6 (with or without Dcr-2Scer\UAS.cUa) has practically no effect on viability.

      Adult escapers expressing wmdKK100974 under the control of Scer\GAL4da.PU show wing defects.

      Adult flies expressing wmdKK100974 (together with Dcr-2Scer\UAS.cUa to enhance RNAi efficiency) under the control of Scer\GAL4elav.PU are short lived and show age-progressive loss of flight ability compared to controls. This decline in flight performance is not observed when the Scer\GAL4Mef2.PR driver is used (without Dcr-2Scer\UAS.cUa).

      (Borg et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      No decline in adult flight ability compared to controls is observed when wmdKK100974 and Gem3ΔN.Scer\UAS are co-expressed under the Scer\GAL4Mef2.PR driver.

      (Borg et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      wmdKK100974
      Name Synonyms
      Secondary FlyBase IDs
        References (3)