Expression of Dph1^KK100975 under the control of Scer\GAL4^esg-NP5130 combined with Gal80[ts] to bypass the developmental stage and Scer\GAL80^Su(H).GBE to limit the expression to intestinal stem cells (ISCs) has no significant effect on the number of ISCs, but it significantly reduced the number of actively proliferating (EdU+) cells in the posterior adult midgut (similar reduction is also observed without the addition of Scer\GAL80^Su(H).GBE).

Expression of Dph1^KK100975 under either Scer\GAL4^GMR11F02 or Scer\GAL4^en.PU does not affect the number of mitotic (pH3+) cells in the third instar larval wing disc and has no impact on the wing size or morphology in adult flies. Scer\GAL4^nos.PG-driven expression (with UAS-Dicer2 to enhance RNAi efficacy) does not induce any loss of germline stem cells in female germaria, compared to controls.