FlyBase Allele Report: Dmel\Raf[KK100982]

General Information

Symbol

Dmel\Raf^KK100982

Species

D. melanogaster

Name

FlyBase ID

FBal0231022

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

P{KK100982}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100982}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100982

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Park et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion rhythm, with Scer\GAL4^phtm.PO

(Selcho et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Raf^KK100982 under the control of Scer\GAL4^phm.PO significantly reduces the circadian rhythmicity of adult emergence after transfer to constant darkness, compared to controls.

(Selcho et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Raf^KK100982, Scer\GAL4^nrv2.PS is a suppressor | partially of abnormal locomotor behavior phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^nrv2.PS

(Park et al., 2013)

Raf^KK100982, Scer\GAL4^nrv2.PS is a suppressor | partially of short lived phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^nrv2.PS

(Park et al., 2013)

Raf^KK100982, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Park et al., 2013)

NOT Suppressor of

Statement

Reference

Raf^KK100982/Scer\GAL4^Dll-md23 is a non-suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Raf^KK100982/Scer\GAL4^Dll-md23 is a non-suppressor | somatic clone of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Raf^KK100982/Scer\GAL4^Dll-md23 is a non-suppressor | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Raf^KK100982/Scer\GAL4^Dll-md23 is a non-suppressor | somatic clone of increased cell number | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Raf^KK100982, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Park et al., 2013)

Raf^KK100982, Scer\GAL4^GMR.PF is a suppressor of photoreceptor phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Park et al., 2013)

NOT Suppressor of

Statement

Reference

Raf^KK100982/Scer\GAL4^Dll-md23 is a non-suppressor | somatic clone of larval brain | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrowth and increased number of progenitor cells characteristic for cno^R2;scrib¹ clones is not significantly suppressed by Scer\GAL4^Dll-md23-driven expression of Raf^KK100982 in the mutant clones.

(Rives-Quinto et al., 2017)

Xenogenetic Interactions

Statement

Reference

Expression of phl^KK100982 partially suppresses the external eye phenotype and photoreceptor degeneration seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF. The progressive impairment of motor performance and reduction in lifespan seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^nrv2.PS are also partially rescued.

(Park et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v107766

P{KK100982}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Raf^KK100982

phl^KK100982

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close