FlyBase Allele Report: Dmel\Dis3[KK101473]

General Information

Symbol

Dmel\Dis3^KK101473

Species

D. melanogaster

Name

FlyBase ID

FBal0231291

Feature type

allele

Associated gene

Dmel\Dis3

Associated Insertion(s)

Carried in Construct

P{KK101473}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101473}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101473

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of multiple myeloma

CEA

(Snee et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr-MD237

(Towler et al., 2015)

haltere, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

wing blade, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

wing disc | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

wing disc | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

wing pouch | third instar larval stage, with Scer\GAL4^nub-AC-62

(Towler et al., 2015)

Detailed Description

Statement

Reference

Expression of Dis3^KK101473 driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs.

Expression of Dis3^KK101473 driven by Scer\GAL4^Bx-MS1096 leads to changes in the mitotic cell cycle, with fewer cells in G1 or S phases and an increased fraction in the G2/M transition phase in third instar larval wing discs compared to wild type.

(Snee et al., 2016)

Expression of Dis3^KK101473 driven by Scer\GAL4^nub-AC-62 results in a complete absence of wing blade development (no wing phenotype) and abnormal haltere in adults with 100% penetrance, and late L3 imaginal discs are significantly smaller than controls and show extensive apoptosis in the wing pouch region. Scer\GAL4^nub-AC-62>Dis3^KK101473 flies show delayed larval development (by 40 hours at 25[o]C). Expression of Dis3^KK101473 driven by Scer\GAL4^pnr-MD237 results in a cleft thorax phenotype in adults with 100% penetrance.

(Towler et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dis3^KK101473, Scer\GAL4^Bx-MS1096 has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by Ras85D^V12.UAS, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Enhancer of

Statement

Reference

Dis3^KK101473, Scer\GAL4^Bx-MS1096 is an enhancer of increased cell growth rate | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Dis3^KK101473, Scer\GAL4^dpp.PU is an enhancer of increased cell growth rate | larval stage phenotype of Raf^UAS.F179, Scer\GAL4^dpp.PU

(Snee et al., 2016)

Dis3^KK101473, Scer\GAL4^Bx-MS1096 is an enhancer of increased cell growth rate | larval stage phenotype of Raf^UAS.F179, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Dis3^KK101473, Scer\GAL4^dpp.PU is an enhancer of increased cell growth rate | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^dpp.PU

(Snee et al., 2016)

Suppressor of

Statement

Reference

Dis3^KK101473, Scer\GAL4^Bx-MS1096 is a suppressor of abnormal mitotic cell cycle | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dis3^KK101473, Scer\GAL4^Bx-MS1096 has wing disc | larval stage phenotype, suppressible by Ras85D^V12.UAS, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Enhancer of

Statement

Reference

Dis3^KK101473, Scer\GAL4^dpp.PU is an enhancer of eye-antennal disc | larval stage phenotype of Raf^UAS.F179, Scer\GAL4^dpp.PU

(Snee et al., 2016)

Dis3^KK101473, Scer\GAL4^dpp.PU is an enhancer of wing disc | larval stage phenotype of Raf^UAS.F179, Scer\GAL4^dpp.PU

(Snee et al., 2016)

Dis3^KK101473, Scer\GAL4^Bx-MS1096 is an enhancer of wing disc | larval stage phenotype of Raf^UAS.F179, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Dis3^KK101473, Scer\GAL4^dpp.PU is an enhancer of eye-antennal disc | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^dpp.PU

(Snee et al., 2016)

Dis3^KK101473, Scer\GAL4^dpp.PU is an enhancer of wing disc | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^dpp.PU

(Snee et al., 2016)

NOT Enhancer of

Statement

Reference

Dis3^KK101473, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing disc | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Suppressor of

Statement

Reference

Dis3^KK101473, Scer\GAL4^Bx-MS1096 is a suppressor of wing disc | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Bx-MS1096

(Snee et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Dis3^KK101473 significantly enhances tissue overgrowth in eye-antennal imaginal or wing discs with expression of Raf^{Scer\UAS.F179} or Ras85D^V12.Scer\UAS driven by Scer\GAL4^dpp.PU (or in wing discs driven by Scer\GAL4^Bx-MS1096).

Co-expression of Dis3^KK101473 and Ras85D^Scer\UAS.cKa or Raf^{K497M.Scer\UAS} driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs (or in wing discs driven by Scer\GAL4^Bx-MS1096).

Co-expression of Dis3^KK101473 and Ras85D^V12.Scer\UAS driven by Scer\GAL4^Bx-MS1096 leads to mutual suppression of mitotic cell cycle changes in third instar larval wing discs with expression of either construct alone.

(Snee et al., 2016)

Xenogenetic Interactions

Statement

Reference

Co-expression of Dis3^KK101473 and Mmmm\ras^N17.Scer\UAS driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs.

(Snee et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v108013

P{KK101473}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Dis3^KK101473

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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