FlyBase Allele Report: Dmel\wap[KK102794]

General Information

Symbol

Dmel\wap^KK102794

Species

D. melanogaster

Name

FlyBase ID

FBal0232012

Feature type

allele

Associated gene

Dmel\wap

Associated Insertion(s)

Carried in Construct

P{KK102794}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK102794}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102794

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Degoutin et al., 2013)

abnormal size | adult stage, with Scer\GAL4^hh.PU

(Degoutin et al., 2013)

abnormal size | adult stage, with Scer\GAL4^rn.PU

(Degoutin et al., 2013)

decreased size | adult stage, with Scer\GAL4^da.PU

(Yang et al., 2016)

partially lethal - majority live, with Scer\GAL4^elav.PU

(Morriss et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

A slight increase in adult eye size is observed when wap^KK102794 is expressed under the control of Scer\GAL4^GMR.PU compared to controls.

Expression of wap^KK102794 under the control of Scer\GAL4^rn.PU results in a 21% decrease in wing size compared to controls.

Flies expressing wap^KK102794 under the control of Scer\GAL4^hh.PU have legs that are 9% shorter than controls.

(Degoutin et al., 2013)

Expression of wap^KK102794 under the control of Scer\GAL4^αTub84B.PL results in pupal stage lethality. The pupal stage tergal depressor of the trochanter (TDT) muscle is either reduced or absent. Some flies exhibit both phenotypes, with a reduced jump muscle on one side of the thorax and a missing jump muscle on the contralateral side.

When wap^KK102794 is expressed under the control of Scer\GAL4^da.G32 lethality occurs at two days post-eclosion. The tergal depressor of the trochanter (TDT) muscle is either reduced or absent. No wing phenotypes are seen, but many eclosed adults fail to expand their wings prior to death.

Flies expressing wap^KK102794 under the control of either Scer\GAL4^elav.PU or both Scer\GAL4^elav.PU and Scer\GAL4¹¹⁵¹ are semi-lethal and those that do eclose die within two days.

Expression of wap^KK102794 under the control of Scer\GAL4¹¹⁵¹ results in tergal depressor of the trochanter (TDT) muscles with a reduced number of fibres compared to controls. A similar, but weaker, phenotype is seen when wap^KK102794 is expressed under the control of Scer\GAL4^Mef2.PR. A significant, but less severe, loss of fibres is also seen when wap^KK102794 is expressed under the control of Scer\GAL4^elav.PU.Expressing wap^KK102794 under the control of Scer\GAL4¹¹⁵¹ and Scer\GAL4^elav.PU simultaneously produces a phenotype that is not more severe than with Scer\GAL4¹¹⁵¹ alone.

(Morriss et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^da.PU, wap^KK102794 has decreased size | adult stage phenotype, suppressible | partially by Scer\GAL4^da.PU/cic^GD1404

(Yang et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^rn.PU/wap^KK102794 is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^rn.PU, mnb^UAS.cUa

(Degoutin et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^da.PU, wap^KK102794 has adult brain phenotype, suppressible | partially by Scer\GAL4^da.PU/cic^GD1404

(Yang et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^rn.PU/wap^KK102794 is a suppressor | partially of wing phenotype of Scer\GAL4^rn.PU, mnb^UAS.cUa

(Degoutin et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wap^KK102794 partially suppresses the wing overgrowth seen when mnb^Scer\UAS.cUa is expressed under the control of Scer\GAL4^rn.PU.

(Degoutin et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of wap^Scer\UAS.cDa rescues the reduction in wing size seen when wap^KK102794 is expressed under the control of Scer\GAL4^rn.PU.

(Degoutin et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v107076

P{KK102794}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG14614^KK102794

wap^KK102794

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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