FlyBase Allele Report: Dmel\Asciz[KK103770]

General Information

Symbol

Dmel\Asciz^KK103770

Species

D. melanogaster

Name

FlyBase ID

FBal0232521

Feature type

allele

Associated gene

Dmel\Asciz

Associated Insertion(s)

Carried in Construct

P{KK103770}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK103770}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK103770

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^en.PU

(Zaytseva et al., 2014)

increased occurrence of cell division | adult stage, with Scer\GAL4^en.PU

(Zaytseva et al., 2014)

lethal - all die during first instar larval stage, with Scer\GAL4^Tub.PU

(Zaytseva et al., 2014)

visible, with Scer\GAL4^en.PU

(Zaytseva et al., 2014)

Phenotype Manifest In

wing, with Scer\GAL4^en.PU

(Zaytseva et al., 2014)

wing blade posterior compartment, with Scer\GAL4^en.PU

(Zaytseva et al., 2014)

wing disc | somatic clone, with Scer\GAL4^Act.PU

(Zaytseva et al., 2014)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Zaytseva et al., 2014)

Detailed Description

Statement

Reference

Expression of Asciz^KK103770 under the control of Scer\GAL4^en.PU results in shrunken and curved wings in which the posterior compartment (the region of Scer\GAL4^en.PU expression) is significantly reduced compared to the anterior compartment. The flies also show a "held-out wings" phenotype.

Mitotic index is increased in the posterior compartment (compared to the anterior compartment) of the wing disc of animals expressing Asciz^KK103770 under the control of Scer\GAL4^en.PU. Mitotic figures show a reduction in prophase and a pronounced increase in anaphase. The total proportion of cells in metaphase is not altered, but the proportion of cells containing a marker for early metaphase is increased, suggesting that mitotic delay starts during late metaphase. Severe spindle alignment and chromosome alignment defects are seen in the dividing cells. Cells are seen extruded from the basal surface of the wing disc and an increase in the number of apoptotic cells is seen.

Clones in the wing disc expressing Asciz^KK103770 under the control of Scer\GAL4^Act.PU are reduced in size compared to control clones.

(Zaytseva et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Asciz^KK103770, Scer\GAL4^en.PU has visible phenotype, suppressible by ctp^UAS.cBa, Scer\GAL4^en.PU

(Zaytseva et al., 2014)

Asciz^KK103770, Scer\GAL4^en.PU has abnormal mitotic cell cycle phenotype, suppressible by ctp^UAS.cBa, Scer\GAL4^en.PU

(Zaytseva et al., 2014)

Asciz^KK103770, Scer\GAL4^en.PU has increased cell death phenotype, suppressible by ctp^UAS.cBa, Scer\GAL4^en.PU

(Zaytseva et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Asciz^KK103770, Scer\GAL4^en.PU has wing disc posterior compartment phenotype, suppressible by ctp^UAS.cBa, Scer\GAL4^en.PU

(Zaytseva et al., 2014)

Asciz^KK103770, Scer\GAL4^en.PU has wing blade posterior compartment phenotype, suppressible by ctp^UAS.cBa, Scer\GAL4^en.PU

(Zaytseva et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ctp^Scer\UAS.cBa suppresses the reduction in size of the posterior compartment of the wing (relative to the anterior compartment) which is seen in flies expressing Asciz^KK103770 under the control of Scer\GAL4^en.PU.

The increased mitotic index seen in the posterior compartment (compared to the anterior compartment) of the wing disc of animals expressing Asciz^KK103770 under the control of Scer\GAL4^en.PU is suppressed by co-expression of ctp^Scer\UAS.cBa. The increase in the number of apoptotic cells is also suppressed.

(Zaytseva et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v100118

P{KK103770}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Asciz^KK103770

CG14962^KK103770

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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