FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ppkKK104185
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General Information
Symbol
Dmel\ppkKK104185
Species
D. melanogaster
Name
FlyBase ID
FBal0232800
Feature type
allele
Associated gene
Dmel\ppk
Associated Insertion(s)
Carried in Construct
P{KK104185}
Also Known As
UAS-ppk-RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK104185}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK104185
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Third instar larvae expressing ppkKK104185 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4ppk.1.9 display hypersensitive behavioral response (shortened latency till response to 42[o]C thermal probe stimulus) in a thermal nociception assay. ppkKK104185 RNAi expression also leads to significantly decreased dendrite coverage in the ddaC dorsal multidendritic neurons compared to controls.

      (Honjo et al., 2016)

      The percentage of third instar larvae with expression of ppkKK104185 driven by Scer\GAL4ppk26.PG showing rolling behavior in response to nociceptive mechanical stimuli (2.5, 5, 12 and 47mN) is significantly reduced compared to wild type.

      (Guo et al., 2014)

      Scer\GAL4ppk.PG-mediated expression of ppkKK104185 results in larvae with a severe mechanical nociception response deficit.

      (Kim et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4ppk.PG-mediated co-expression of ppkKK104185 and PiezoNIG.8486R results in larvae with a more severe mechanical nociception response deficit (nearly complete abolishment of response) than expression of either ppkKK104185 or PiezoNIG.8486R alone. Co-expressing larvae are indistinguishable from wild type in assays for responses to high temperature or innocuous gentle touch.

      (Kim et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ppkKK104185
      (Mauthner et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)