FlyBase Allele Report: Dmel\Nf-YB[KK107815]

General Information

Symbol

Dmel\Nf-YB^KK107815

Species

D. melanogaster

Name

FlyBase ID

FBal0235184

Feature type

allele

Associated gene

Dmel\Nf-YB

Associated Insertion(s)

Carried in Construct

P{KK107815}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK107815}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK107815

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

increased cell death | third instar larval stage, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

eye disc posterior to the morphogenetic furrow, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

interommatidial bristle, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

ommatidium, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

photoreceptor cell R7 | third instar larval stage, with Scer\GAL4^GMR.PS

(Ly et al., 2013)

Detailed Description

Statement

Reference

Expression of Nf-YB^KK107815 mediated by Scer\GAL4^Act5C.PU, Scer\GAL4^ey.PU, Scer\GAL4^en.PU or Scer\GAL4^pnr-MD237 results in no detectable phenotype.

Expression of one copy of Nf-YB^KK107815 via Scer\GAL4^GMR.PS results in abnormal eye morphology, exhibited as a rough eye. Phenotypic severity increases when two copies of Nf-YB^KK107815 are expressed: many ommatidia lack their hexagonal shape and bristles are missing.

Scer\GAL4^GMR.PS Nf-YB^KK107815 eye imaginal discs show a significant increase in cell death signals in the region posterior to the morphogenetic furrow.

In contrast to wild type, eye discs carrying Scer\GAL4^GMR.PS and two copies of Nf-YB^KK107815 display BrdU incorporation posterior to the second mitotic wave, indicating a delay in S phase and/or ectopic S phases. This DNA synthesis is followed by delayed/ectopic cell division.

Scer\GAL4^GMR.PS-mediated expression of Nf-YB^KK107815 specifically inhibits differentiation of R7 photoreceptor cells in eye imaginal discs.

(Ly et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nf-YB^KK107815, Scer\GAL4^GMR.PS has increased cell death | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Ly et al., 2013)

Nf-YB^KK107815, Scer\GAL4^GMR.PS has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Ly et al., 2013)

Nf-YB^KK107815, Scer\GAL4^GMR.PS has increased occurrence of cell division | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Ly et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nf-YB^KK107815, Scer\GAL4^GMR.PS has eye phenotype, suppressible by Raf^hs.PU

(Ly et al., 2013)

Nf-YB^KK107815, Scer\GAL4^GMR.PS has eye phenotype, suppressible by sev^hs.PU

(Ly et al., 2013)

Nf-YB^KK107815, Scer\GAL4^GMR.PS has photoreceptor cell R7 | third instar larval stage phenotype, suppressible by Raf^hs.PU

(Ly et al., 2013)

Nf-YB^KK107815, Scer\GAL4^GMR.PS has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Ly et al., 2013)

Nf-YB^KK107815, Scer\GAL4^GMR.PS has eye disc posterior to the morphogenetic furrow phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Ly et al., 2013)

NOT suppressed by

Statement

Reference

Nf-YB^KK107815, Scer\GAL4^GMR.PS has photoreceptor cell R7 | third instar larval stage phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Ly et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of phl^hs.PU or sev^hs.PU rescues the Scer\GAL4^GMR.PS Nf-YB^KK107815 rough eye phenotype.

Co-expression of phl^hs.PU restores R7 photoreceptor differentiation in Scer\GAL4^GMR.PS Nf-YB^KK107815 eye discs.

(Ly et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa completely rescues the Scer\GAL4^GMR.PS Nf-YB^KK107815 rough eye phenotype and the increased eye imaginal disc apoptosis.

Co-expression of BacA\p35^Scer\UAS.cHa significantly reduces the ectopic/delayed BrdU incorporation and cell division seen in Scer\GAL4^GMR.PS Nf-YB^KK107815 eye discs.

Co-expression of BacA\p35^Scer\UAS.cHa does not rescue the lack of R7 photoreceptor differentiation in Scer\GAL4^GMR.PS Nf-YB^KK107815 eye discs.

(Ly et al., 2013)

Complementation and Rescue Data

Partially rescued by

Nf-YB^KK107815 is partially rescued by Scer\GAL4^GMR.PS/Nf-YB^{UAS.Tag:polyHis}

(Ly et al., 2013)

Comments

Co-expression of Nf-YB^{Scer\UAS.T:Zzzz\His6} at least partially rescues the rough eye phenotype of Nf-YB^KK107815 Scer\GAL4^GMR.PS flies.

(Ly et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103655

P{KK107815}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nf-YB^KK107815

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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