FlyBase Allele Report: Dmel\Syt1[KK108653]

General Information

Symbol

Dmel\Syt1^KK108653

Species

D. melanogaster

Name

FlyBase ID

FBal0235713

Feature type

allele

Associated gene

Dmel\Syt1

Associated Insertion(s)

Carried in Construct

P{KK108653}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108653}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108653

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

increased body size | third instar larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

majority die during larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

partially lethal - majority die, with Scer\GAL4^ETH.Switch

(Park et al., 2014)

partially lethal - majority die, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Syt1^KK108653 (along with UAS-Dcr-2 expression) driven by Scer\GAL4^phm.PO leads to developmental delay/arrest at the larval stage (around 50% at L1/2 and 30% at L3). Arrested third instar larvae also show an overgrowth phenotype due to the extended larval feeding period. Those larvae that do initiate pupariation are significantly delayed in doing so and exhibit overgrowth phenotypes. Feeding ecdysone (E) to larvae almost fully rescues the phenotypes.

(Yamanaka et al., 2015)

Expression of Syt1^KK108653 RNAi under the control of Scer\GAL4^ETH.Switch results in significantly reduced developmental viability.

(Park et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Syt1^KK108653 is a suppressor | partially of majority die during larval stage phenotype of Gαq^Q203L.UAS, Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Itp-r83A^GD1676, Unc-13-4B^GD7394, Rab3^KK108633 or Syt1^KK108653 (along with UAS-Dcr-2 expression) significantly suppresses larval lethality seen in Scer\GAL4^phm.PO>Gαq^{Q203L.Scer\UAS}.

(Yamanaka et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v100608

P{KK108653}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Syt1^KK108653

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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