FlyBase Allele Report: Dmel\PICK1[KK109273]

General Information

Symbol

Dmel\PICK1^KK109273

Species

D. melanogaster

Name

FlyBase ID

FBal0236189

Feature type

allele

Associated gene

Dmel\PICK1

Associated Insertion(s)

Carried in Construct

P{KK109273}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK109273}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK109273

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(McGurk and Bonini, 2012)

ameliorates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(McGurk and Bonini, 2012)

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(McGurk and Bonini, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mef2.PU

(Mallik et al., 2017)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Mef2.PU

(Mallik et al., 2017)

NMJ bouton | third instar larval stage, with Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NMJ bouton | third instar larval stage, with Scer\GAL4^Mef2.PU

(Mallik et al., 2017)

Detailed Description

Statement

Reference

Expression of PICK1^KK109273 under the control of either Scer\GAL4^Act5C.PU or Scer\GAL4^Mef2.PU results in smaller synapse size and reduced number of boutons on neuromuscular junctions in third instar larvae compared to controls.

(Mallik et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

PICK1^KK109273, Scer\GAL4^Act5C.PU has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NOT Enhancer of

Statement

Reference

PICK1^KK109273, Scer\GAL4^Act5C.PU is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Suppressor of

Statement

Reference

PICK1^KK109273, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

PICK1^KK109273, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

PICK1^KK109273, Scer\GAL4^Act5C.PU has NMJ bouton | third instar larval stage phenotype, non-enhanceable by ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

PICK1^KK109273, Scer\GAL4^Act5C.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

NOT Enhancer of

Statement

Reference

PICK1^KK109273, Scer\GAL4^Act5C.PU is a non-enhancer of NMJ bouton | third instar larval stage phenotype of ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

PICK1^KK109273, Scer\GAL4^Act5C.PU is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of ICA69^GD7020, Scer\GAL4^Act5C.PU

(Mallik et al., 2017)

Suppressor of

Statement

Reference

PICK1^KK109273, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

PICK1^KK109273, Scer\GAL4^ninaE.PT is a suppressor of eye photoreceptor cell phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^ninaE.PT

(McGurk and Bonini, 2012)

PICK1^KK109273, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The neuromuscular junction structural defects (smaller synapse, reduced number of boutons) characteristic for third instar larvae expressing either PICK1^KK109273 or ICA69^GD7020 under the control of Scer\GAL4^Act5C.PU are not further worsened when the two RNAi alleles are co-expressed.

(Mallik et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v104486

P{KK109273}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

PICK1^KK109273

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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