Expression of AP-1-2βJF02963 under the control of Scer\GAL4ey.PU induces a headless phenotype in pupae. Expression under the control of Scer\GAL4elav-C155 or Scer\GAL4Toll-6-D42 results in active larvae that die as early pupae. Expression under the control of Scer\GAL4elav-C155 (in combination with Dicer-2 for efficient RNAi) also leads to a significant increase in the number of boutons at the larval muscle 6/7 NMJs, as compared to controls.
Under starvation conditions, the expression of AP-1-2βJF02963 under the control of Scer\GAL4Cg.PA results in the third instar larval fat body showing an the accumulation and enlargement of early and late endosomes (identified as GFP-FYVE and GFP-Rab7 cytoplasmic puncta, respectively), an accumulation of lysosomes at the cell membrane (identified as LAMP-GFP) , and an accumulation and enlargement of autophagosomes (identified as GFP-Atg8 cytoplasmic puncta and p62 protein cytoplasmic puncta), as compared to starved controls. However, similar defects in early and late endosomes, lysosomes and autophagosomes are also observed under normal diet.