FlyBase Allele Report: Dmel\Hr51[X1]

General Information

Symbol

Dmel\Hr51^X1

Species

D. melanogaster

Name

FlyBase ID

FBal0239784

Feature type

allele

Associated gene

Dmel\Hr51

Associated Insertion(s)

Carried in Construct

Also Known As

unf^X1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Sung et al., 2009)

ends-in gene targeting

(Sung et al., 2009)

Progenitor genotype

Cytology

Description

Hr51^X1 carries a mutation in the second exon.

(Yaniv et al., 2012)

Mutation of the first base of Hr51 intron 2 from a "g" to a "C", which is predicted to destroy the 5' donor splice site of intron 2 and to result in an in-frame stop codon, producing a truncated protein of 145 amino acid residues that lacks the second zinc finger, the hinge region and the ligand-binding domain. In addition, bases 5 and 6 of the endogenous intron 2 sequence have been mutated and 4 bases inserted to produce an XbaI site.

(Sung et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:15,333,968..15,333,968 [+]

Nucleotide change:

G15333968C

Comment:

Splice donor mutation in the first base of intron two. An XbaI site was also introduced. Created by homologous recombination.

(Sung et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm (with Df(2R)ED2426)

(Beuchle et al., 2012)

abnormal locomotor rhythm (with Hr51^Z0001)

(Beuchle et al., 2012)

abnormal locomotor rhythm | recessive

(Beuchle et al., 2012)

abnormal neuroanatomy | somatic clone

(Yaniv et al., 2012)

female sterile (with Df(2R)ED2426)

(Sung et al., 2009)

male sterile (with Df(2R)ED2426)

(Sung et al., 2009)

visible (with Df(2R)ED2426)

(Sung et al., 2009)

visible (with Hr51^Z0001)

(Sung et al., 2009)

visible | recessive

(Sung et al., 2009)

Phenotype Manifest In

alpha/beta Kenyon cell | somatic clone

(Yaniv et al., 2012)

gamma Kenyon cell | somatic clone

(Yaniv et al., 2012)

lobe system of adult mushroom body

(Bates et al., 2010)

lobe system of adult mushroom body (with Df(2R)ED2426)

(Bates et al., 2010)

lobe system of adult mushroom body (with Hr51^Z0001)

(Bates et al., 2010)

wing

(Sung et al., 2009)

wing (with Df(2R)ED2426)

(Sung et al., 2009)

wing (with Hr51^Z0001)

(Sung et al., 2009)

Detailed Description

Statement

Reference

Homozygous Hr51^X1 mutant flies exhibit a somewhat unusual but diurnal activity pattern in LD conditions (12 hours light: 12 hours dark) that have a delayed morning activity peak and an advanced evening activity peak. The majority of homozygous Hr51^X1 flies are arrhythmic in DD conditions (constant darkness). No defects are seen in heterozygotes in either LD or DD.

Hr51^X1/Df(2R)ED2426 mutant flies are arrhythmic in both LD conditions (12 hours light: 12 hours dark) and DD (constant darkness).

Hr51^X1/Hr51^Z0001 mutant flies are arrhythmic in both LD conditions (12 hours light: 12 hours dark) and DD (constant darkness).

(Beuchle et al., 2012)

Hr51^X1 mutant neuroblast clones exhibit γ-lobe defects with a decrease in innervation of the adult γ-lobe. α/β neurons exhibit normal morphology and show no signs of axon stalling.

Hr51^X1 heterozygous MARCM clones exhibit guidance defects in α/β neurons.

(Yaniv et al., 2012)

Hr51^X1/Df(2R)ED2426 hemizygous and Hr51^X1 homozygous mutants show a complete loss of mushroom body (MB) lobes. MB axons of adult hemizygous and homozygous mutants project anteroventrally, forming a peduncle, but stall prior to the formation of discrete lobes. Axons that reach the heel region of the MB tend to spread out, but do not extend axons medially or dorsally. γ neurons extend axons medially but not dorsally in Hr51^X1/Df(2R)ED2426 larvae and they undergo pruning like wild-type axons at approximately 16 hours after puparium formation (APF). However, they fail to re-extend during pupal development. α'/β' and α/β projections are never observed in Hr51^X1/Df(2R)ED2426 pupae at 48 hours APF.

Hr51^X1/Hr51^Z0001 heterozygotes exhibit a range of mushroom body (MB) phenotypes, for example, missing MB lobes.

(Bates et al., 2010)

Hr51^Z0001/Df(2R)ED2426 can show reduced eclosion, although the phenotype is variable in different crosses.

Approximately a quarter of Hr51^X1/Df(2R)ED2426 adults have unexpanded wings.

44% of homozygous flies fail to eclose and 17% eclose but have unexpanded wings.

15% of Hr51^X1/Hr51^Z0001 flies fail to eclose and 62% eclose but have unexpanded wings.

(Sung et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Hr51^Z0001

(Sung et al., 2009)

Partially rescued by

Hr51^X1/Df(2R)ED2426 is partially rescued by Hr51^{+tCH322-72C06}

(Beuchle et al., 2012)

Hr51^X1/Df(2R)ED2426 is partially rescued by Scer\GAL4^ey-OK107/Hr51^UAS.cBa

(Bates et al., 2010)

Hr51^X1/Df(2R)ED2426 is partially rescued by Hr51^cSa

(Sung et al., 2009)

Comments

Expression of Hr51^+tCH322-72C6 partially rescues the locomotor rhythm defects seen in Hr51^X1/Df(2R)ED2426 mutants. Free-running rhythms are restored in 80% of flies.

(Beuchle et al., 2012)

Medial and dorsal mushroom body (MB) lobes are observed in all Hr51^X1/Df(2R)ED2426 animals expressing Hr51^Scer\UAS.cBa driven by Scer\GAL4^ey-OK107, but MB lobes appear thin and less robust in some rescued animals. The medially projecting axons in rescued animals fail to stop appropriately, extending axons past the midline.

(Bates et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

56835

w¹¹¹⁸; Hr51^X1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hr51^X1

unf^X1

(Beuchle et al., 2012, Yaniv et al., 2012, Bates et al., 2010, Sung et al., 2009)

unf^x1

(Kozlov et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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