FlyBase Allele Report: Dmel\mir-184[Δ]

General Information

Symbol

Dmel\mir-184^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0239919

Feature type

allele

Associated gene

Dmel\mir-184

Associated Insertion(s)

Carried in Construct

Also Known As

Δmir-184

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Iovino et al., 2009)

Mutagen

FLPase

(Iovino et al., 2009)

Progenitor genotype

PBac{WH}f05119

(Iovino et al., 2009)

P{XP}d08710

(Iovino et al., 2009)

Cytology

Description

Recombination between the two progenitor insertions has resulted in a deletion that removes the sequence between them, including the mir-184 gene.

(Iovino et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:13,310,903..13,332,903 [-]

Comment:

An FLP-induced 22kb deletion between PBac{WH}f05119 and P{XP}d08710. The breakpoint at 2R:9198408 is based on the genomic position of PBac{WH}f05119. The breakpoint at 2R:9220408 is approximate since the genomic location of P{XP}d08710 was not reported.

(Iovino et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of major depressive disorder

CEA

(Mendes-Silva et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity | female

(Iovino et al., 2009)

lethal - all die during embryonic stage | maternal effect | recessive

(Iovino et al., 2009)

male fertile

(Iovino et al., 2009)

some die during embryonic stage | recessive | maternal effect | recessive

(Iovino et al., 2009)

viable

(Iovino et al., 2009)

Phenotype Manifest In

dorsal appendage | maternal effect

(Iovino et al., 2009)

egg | maternal effect

(Iovino et al., 2009)

ovariole

(Iovino et al., 2009)

ovary

(Iovino et al., 2009)

Detailed Description

Statement

Reference

Homozygous adults are viable and appear morphologically normal.

Homozygous females lay far fewer eggs than normal, and the eggs and embryos they produce have severe abnormalities. 2 to 3 day old females lay 5-10 eggs/day, and approximately 70% of the eggs have normal external morphology and are fertilised. However, 85% of the embryos show severe defects in anteroposterior patterning, many also show severe defects in cellularisation and only approximately 1% of the progeny develop to adulthood. As the females age, egg production declines further and the number of eggs with abnormal external morphology increases. Eggs from 3 to 4 day old homozygous females are typically smaller than normal, and many have defects in dorsoventral patterning of the eggshell (as assayed by the position and length of the dorsal appendages). Homozygous females that are 5 days of age of older lay almost no eggs.

Approximately 85% of embryos from morphologically normal eggs laid by 2 to 3 day old homozygous females mated to homozygous males show severe defects in anteroposterior patterning. In many embryos from young homozygous females mated to homozygous males, the anterior nuclei fail to move to the anterior tip of the embryo and instead remain at approximately 80% egg length, so that although cellularisation subsequently occurs, the anterior tip remains devoid of nuclei and merely fills with yolk. These embryos develop further and undergo gastrulation and germband extension/retraction, but die at approximately stage 13 of embryogenesis/.

Eggs produced by 2 to 4 day old homozygous females mated to homozygous males are often smaller than normal. In addition, 80% of the eggs show defects in dorsoventral patterning of the eggshell; nearly all of these are dorsalised, with the dorsal appendages more widely spaced and shorter than normal, very occasionally, ventralised eggs are seen.

The oocyte nucleus is in its normal position in stage 9 homozygous egg chambers.

The ovarioles of 5 day old homozygous females are filled with germline stem cell-like cells, but no cysts. Many empty ovarioles are also present. The ovaries of older females are completely devoid of any germ cells.

(Iovino et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-184^Δ has decreased fecundity | female phenotype, suppressible | partially by sax⁴/sax[+]

(Iovino et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

One copy of sax⁴ substantially rescues the infertility of mir-184^Δ females; they produce many more eggs and do not become sterile over time.

(Iovino et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

mir-184^Δ is partially rescued by mir-184^UAS.cIa

(Iovino et al., 2009)

mir-184^Δ is partially rescued by mir-184^UAS.cIa/Scer\GAL4^{VP16.nanos.UTR}

(Iovino et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mir-184^Δ

ΔmiR-184

(Eyk et al., 2019, Mendes-Silva et al., 2019)

Δmir-184

(Iovino et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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