FB2026_02 , released June 18, 2026
Allele: Ecol\lacZTg.12231-13046
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General Information
Symbol
Ecol\lacZTg.12231-13046
Species
E. coli
Name
FlyBase ID
FBal0239963
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{Tg-lacZ.12231-13046}
Also Known As
Tg-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Iklé et al., 2008)
Progenitor genotype
Carried in construct
P{Tg-lacZ.12231-13046}
Cytology
Description

A Tg enhancer fragment (+12231 to +13046) from the large intron (primers used to generate the construct: forward primer 5'-GCATATTGCTGCAATTGG, reverse primer 5'-TAAGTATTTTCGGCAGGG) is fused upstream of a minimal promoter driving expression of a lacZ reporter.

(Iklé et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
Tg_cardiac_enhancer
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult ostial pore
      (Shah et al., 2011)
      in situ
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 15
      embryonic/larval pharynx
      (Iklé et al., 2008)
      embryonic/larval heart | restricted
      (Iklé et al., 2008)
      embryonic stage 15 -- 16
      embryonic/larval dorsal vessel | subset
      (Iklé et al., 2008)
      embryonic stage 16
      embryonic cardiac outflow tract cardioblast
      (Iklé et al., 2008)
      larval cardial cell
      (Iklé et al., 2008)
      Additional Information
      Statement
      Reference

      Reporter expression is observed in 5 sets of ostia cells, spanning from the posterior of the thorax to abdominal segment 5.

      (Shah et al., 2011)

      Reporter expression is observed in the pharynx and "Svp" cells at stage 15 and in the cardiac outflow region by stage 16. It is also observed in the cardial cells of the dorsal vessel. Expression was strong in the cardial cells expressing svp but not in those expressing tin.

      (Iklé et al., 2008)
       
      Marker for
      Reflects expression of
      Tg
      (Shah et al., 2011, Iklé et al., 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\lacZTg.12231-13046
      Name Synonyms
      Secondary FlyBase IDs
        References (3)