Amino acid replacement: G532R.
G21080441M
G583R | tadr-PC; G583R | tadr-PD
G532R
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
The rhabdomeres of 1 day old mutant flies are virtually normal. 7 day old mutant flies show a severe decrease in the average size of the peripheral rhabdomeres, and the microvilli of most peripheral rhabdomeres are severely shortened (although they are not detached). 2 week old mutant flies contain detached and broken microvilli in peripheral rhabdomeres, and the overall length of the microvilli is much shorter than normal. The cell bodies of the peripheral rhabdomeres are severely shrunken. The central R7 rhabdomeres contain normally attached microvilli, although many of the rhabdomeres are deformed.
Mutant 7 day old flies raised completely in the dark have some irregularly shaped and/or smaller rhabdomeres, but the phenotype is much milder than mutant flies exposed to light.
Electroretinogram responses of 1 day old mutant flies have a smaller amplitude than those of wild-type flies. Electroretinogram responses of 7 day old mutant flies have a smaller amplitude and slower termination than those of wild-type flies.
In whole-cell current recordings in the dark, mutant photoreceptors have a normal background inward current and reversal potential. The mutant photoreceptors show a smaller light response compared to wild type.
tadr1 has rhabdomere phenotype, suppressible by ninaE5
tadr1 has rhabdomere phenotype, suppressible by Gαq1
The average size of the peripheral rhabdomeres in 7 day old tadr1 ; ninaE5 flies is significantly larger than in tadr1 single mutants and is the same as in ninaE5 single mutants.
The average size of the peripheral rhabdomeres in 7 day old tadr1 ; Arr25 double mutant flies is smaller than that of tadr1 and Arr25 single mutants.
The reduced size of the peripheral rhabdomeres in 7 day old tadr1 flies is suppressed if they are also mutant for Gα49B1.